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    Apoptosis of hippocampus and cerebellum induced with brain ischemia reperfusion prevented by 3',4'-dihydroxyflavonol (DiOHF)
    (Taylor & Francis Ltd, 2024) Daşdelen, Derviş; Solmaz, Merve; Moğulkoç, Rasim; Baltacı, Abdülkerim Kasım; Erdoğan, Ender
    The present study aimed to determine the effect of 3',4'-dihydroxyflavonol (DiOHF) on apoptosis in the cerebellum and hippocampus in rats with ischemia-reperfusion. A total of 38 Wistar albino male rats were used. Experimental groups were designed as Group 1-Sham; Group 2-Ischemia-reperfusion (IR), in which animals were anesthetized and carotid arteries ligated for 30 minutes (ischemia) and reperfused 30 minutes; Group 3- IR + DiOHF (10 mg/kg); Group 4- Ischemia + DiOHF (10 mg/kg) + reperfusion; Group 5-DiOHF + IR. DiOHF was supplemented as 10 mg/kg by intraperitoneal injection 30 minutes before IR. Following application, the animals were sacrificed under general anesthetic by cervical dislocation, and the cerebellum and hippocampus tissues were analyzed for apoptosis. IR significantly increased hippocampus and cerebellum apoptosis activity, confirmed by Hematoxylin-Eosin, TUNEL labeling, and Caspase-8 activity. However, these values were significantly suppressed by the administration of DiOHF, especially when used before the ischemia and reperfusion. The results of the study show that increased apoptosis in the cerebellum and hippocampus tissue was inhibited by intraperitoneal DiOHF supplementation.
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    Öğe
    Naringin affects caspase-3, il-1β, and hif-1α levels in experimental kidney ischemia-reperfusion in rats
    (Bentham Science Publ Ltd, 2024) Danış, Esra Gülsüm; Acar, Gözde; Daşdelen, Derviş; Solmaz, Merve; Moğulkoç, Rasim; Baltacı, Abdulkerim Kasım
    Background Microvascular dysfunction develops in tissues after Ischemia-Reperfusion (I/R). The current study aimed to determine the effect of naringin supplementation on kidney caspase-3, IL-1 beta, and HIF-1 alpha levels and kidney histology in rats undergoing unilateral nephrectomy and kidney-ischemia reperfusion.Methods The study was conducted on 8-12 weeks old 40 Wistar-type male rats. Experimental renal ischemia-reperfusion and unilateral nephrectomy were performed under general anesthesia in rats. Experimental groups were formed as follows: 1-Control group, 2-Sham control + Vehicle group, 3- Renal ischemia-reperfusion (Renal I+R) + Vehicle group, 4-Renal I+R + Naringin (50 mg/kg/day) group (3 days application) group, 5-Renal I+R + Naringin (100 mg/kg/day) group (3 days supplementation). Nephrectomy in the left kidneys and the ischemia for 45 minutes and reperfusion in the right kidneys followed by 72 hours of reperfusion. Naringin was administered intraperitoneally at the beginning of the reperfusion, 24 hours and 48 hours later. At the end of the experiments, blood was first taken from the heart in animals under general anesthesia. Then, the animals were killed by cervical dislocation, and kidney tissue samples were taken. Tissues were evaluated for caspase-3, IL-1 beta, and HIF-1 alpha as well as histologically.Results As a result of ischemia in kidney tissues, HIF-1 alpha decreased, while caspase-3 and IL-1 beta increased. I/R also caused damage to the kidney tissue. However, naringin supplementation corrected the deterioration to a certain extent.Conclusion The results of the study showed that naringin may have protective effects on kidney damage due to anti-inflammatory and antiapoptosis mechanisms caused by unilateral nephrectomy and I/R in rats.