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A case-control study on sh2b1 gene variants in obesity and obstructive sleep apnea severity: genetic risk factors in the leptin signaling pathway
(London, 2025) Serkan Küççüktürk; Şebnem Yosunkaya; Mehmet Ali Karaselek; Şennur Demirel; Hasibe Vural
Background: Obstructive sleep apnea (OSA) is a common sleep disorder, frequently observed in obese individuals, with shared mechanisms involving leptin and its receptor, which regulate appetite and energy expenditure. SH2B1 is a key enhancer of signaling in the leptin receptor pathway. This study aimed to investigate the association between SH2B1 variants and OSA. Research design and methods: This case-control study included 160 male patients with OSA and 76 healthy controls, stratified into subgroups based on BMI (≤25 kg/m2 and ≥ 30 kg/m2). Polysomnography and anthropometric measurements were performed, and genotyping of three SH2B1 variants (rs7498665, rs4788102, and rs7359397) was conducted. Results: Mutant genotypes of all three SH2B1 variants were significantly associated with higher BMI. Additionally, normal genotypes of rs4788102 and rs7359397 were associated with higher apnea-hypopnea index (AHI) values, indicating a potential risk for OSA. Conclusion: The findings suggest that while SH2B1 variants are strongly associated with BMI, specific normal genotypes may independently contribute to OSA risk by increasing AHI values.
The protective effect of curcumin on the diabetic uterus: Quantitative and qualitative evaluation
(Elsevier Ltd., 2025) Mutlu, Ayşenur Kaya; Tüfekci, Kıymet Kübra; Kaplan, Süleyman
Background: The objective of this study was to examine the impact of diabetes on the rat uterus and to assess the potential therapeutic benefits of curcumin in a diabetic uterus. Materials and methods: A total of thirty-eight female Wistar albino rats were randomly assigned to seven experimental groups. The control group (Cont) was not subjected to any treatment. The sham group (Sham) was administered corn oil, while the curcumin group (Curc) received 30 mg/kg curcumin. A single dose of 50 mg/kg streptozotocin (STZ) was administered to induce experimental diabetes. The diabetic animals were then divided into four groups: a group with diabetes mellitus (DM), a group administered curcumin after seven days of diabetes induced (DC1) and 21 days (DC2) after the onset of diabetes, and a group that received curcumin simultaneously with STZ (DC3). The Cavalieri's method was used to estimate the volume ratios of the uterine epithelium, layers, and blood vessels. Results: The volume ratio of the myometrium was observed to be higher in the DC2 group than in the Cont and DM groups. Conversely, the endometrial volume ratio was found to be lower in the DC2 group than in the Cont group. The cell borders and basement membranes of the epithelial and gland cells were well preserved in the curcumin treatment groups, despite the obvious damage observed in the DM group. Similar findings were also observed in the electron microscopic sections, with collagen fibers, which were arranged in thick bands in the DM group and were unable to maintain their ultrastructure, were well organized in the DC1, DC2, and DC3. Conclusion: Considering that it improves the endometrial structure and reduces degeneration in the surface and gland epithelium, it can be said that curcumin is an effective agent in reducing and preventing complications associated with DM in the uterus.
Exploring the anticancer potential of dianthus orientalis in pancreatic cancer: a molecular and cellular study
(Elsevier Ltd, 2025) Yıldırım, Mehmet Aykut; Sevinç, Burak; Paydaş, Samet; Karaselek, Mehmet Ali; Duran, Tuğçe; Küççüktürk, Serkan
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies, characterized by limited therapeutic options and poor prognosis. This study investigated the anticancer effects of Dianthus orientalis extracts on pancreatic cancer cell lines (Panc-1 and BxPC-3) with a focus on apoptosis, invasion, and metastasis-related pathways. Chemical analysis using HPLC-MS/MS identified bioactive compounds (vanillic acid, syringic acid, p-coumaric acid, quercetin, hyperoxide) known for their anticancer properties. The Inhibitory Concentration 50 (IC50) value, which is half maximal inhibitory concentration of cancer cells, was determined to be 250 μg/mL at 48 h, and significantly induced apoptosis by decreasing anti-apoptotic gene expressions (Bcl-XL and Bcl-2) and increasing pro-apoptotic gene expressions (Casp3 and Bax) particularly in the Panc-1 cell line. Flow cytometric analysis revealed approximately 55% apoptosis in both cell lines. Molecular docking and dynamics analyses demonstrated strong binding affinities of quercetin and hyperoxide with important cancer-related proteins, including CHEK2 and PALB2. Gene expression analysis demonstrated the upregulation of pro-apoptotic markers like Bax and Casp3, and selective modulation of metastasis-related genes such as SNAI1, PALB2, and CHEK2. The findings highlight the potential of D. orientalis to modulate DNA repair mechanisms and epithelial-mesenchymal transition (EMT) pathways. Furthermore, network pharmacology and in silico tools corroborated its interactions with critical signaling pathways in cancer progression, including PI3K-Akt and MAPK. The study provides the first comprehensive evidence of D. orientalis's antiproliferative through apoptosis induction on pancreatic cancer cell lines, laying the groundwork for future therapeutic applications. © 2025 Elsevier Ltd
Comparison of renal tubular damage with kidney ınjury molecule-1 in open and laparoscopic colorectal cancer surgery
(Kaunas : Lietuvos gydytojų sąjunga, 2024) Duyan, Abdullah Gürhan; Vatansev, Celalettin; Kocabaş, Rahim; Koç, Melek Yalçın; Akbulut, Muhammed Ai
Background and Objectives: Colorectal cancer is the third most common type of cancer in men and women. With advancements in technology, minimally invasive treatment options have become increasingly prominent in colorectal cancer surgery. This study aimed to compare the increased intra-abdominal pressure in laparoscopic colon and rectal surgery with open procedures using kidney injury molecule-1 (KIM-1) secreted from renal tubules. Materials and Methods: We enrolled 46 patients diagnosed with colon cancer who underwent laparoscopic and open surgical procedures at our clinic. The patients were prospectively randomized into five groups: 10 laparoscopic right hemicolectomies (Group 1), 8 open right hemicolectomies (Group 2), 8 laparoscopic anterior resections (LARs) (Group 3), 11 open anterior resections (Group 4), and 9 laparoscopic low anterior resections (Group 5). Urine samples were collected from the patients preoperatively, postoperatively at the 4th hour, and postoperatively on the 14th day, and the urine KIM-1 levels and urine creatinine (Cr) values were measured. The urine KIM-1/Cr ratios were subsequently calculated. Results: The urinary KIM-1/Cr levels increased at the 4th postoperative hour after the open and laparoscopic procedures. On postoperative day 14, the urinary KIM-1/Cr levels were lower than those in the preoperative period in all groups, except the LAR group. Conclusions: Our study shown that the average pressure in laparoscopic colon and rectal surgery did not have a long-term impact on kidney injury in comparison to open colon and rectal surgery.
Morphometry of latissimus dorsi in fetal cadavers for prenatal spina bifida surgery
(Turkısh Neurosurgıcal Soc, 2025) Özdemir, Berfu; Dursun, Ahmet; Kastamoni, Yadigar; Ayazoğlu, Mehtap; Şanlı Onur Can; Albay, Soner
AIM: To investigate latissimus dorsi morphometry, innervation, vascularization, and variational situations in fetuses. MATERIAL and METHODS: Forty-nine fetuses, aged between 15 and 40 weeks of gestation, were examined for the morphological development of the latissimus dorsi. The horizontal lengths of the thoracolumbar fascia, the dimensions of the latissimus dorsi and its tendon were measured. Additionally, the attachments of the latissimus dorsi and the branching pattern of the thoracodorsal nerve were evaluated. The development of all measured parameters during gestational age (trimester) and their interrelationships were assessed. Data were compared across sides (right and left) and genders. RESULTS: The study found that the increase in all parameters was proportional to gestational age. No significant differences were observed between sides or genders. The latissimus dorsi was directly attached (via muscle fiber) to the iliac crest in 60.2% of cases. An 88.5% attachment rate to the inferior angle of the scapula was noted, with 4.1% of these attachments being muscular. Additionally, 9.8% of latissimus dorsi tendons were found to adhere to the teres major tendon. The thoracodorsal nerve was observed to divide into 2 to 8 branches before entering the latissimus dorsi. CONCLUSION: We believe this study will provide valuable insights into the development of intrauterine invasive fetal procedures for tendon, muscle, and nerve transfer repair and assist in determining the most appropriate timing for intervention.
The appearance of external genital organs and anogenital distance in male fetal cadavers
(Springer, 2025) Özgüner, Gülnur; Öztürk, Kenan; Dursun, Ahmet; Bilkay, Cemil; Sulak, Osman
This study aimed to determine the normal size of the male external genital organs and anogenital distance in human fetuses during the fetal period through the anatomic morphometric method. The study was performed on 104 spontaneously aborted human male fetuses aged between 10 and 39 weeks of gestation. Fetuses were divided into groups according to gestational weeks, months, and trimesters. Parameters belonging to the male external genital organs were measured, including penile length and width, transverse scrotal diameter, anterior-posterior scrotal diameter, the distance from the anterior and posterior aspect of the penis to the center of the anus, and the anogenital distance. The mean of each parameter was computed by gestational weeks, months, and trimester groups, and data were presented as mean +/- standard deviation. The mean of each parameter increased with gestational age, and statistically significant differences were observed between trimester groups. A high correlation was also determined between gestational age and the parameters measured.
The effect of endurance training on cardiac and muscle damage in male football players
(Mre Press, 2024) Gündüz, Kerem; Nas, Kazım; Arıcı, Hasan; Pamuk, Ömer
This study aimed to investigate the effects of a six-week endurance training program on heart and muscle damage in football players. Twenty male football players from the same team voluntarily participated in the study. Participants were randomly divided into two groups: the experimental group (n = 10; mean age 22 +/- 2.21 years) and the control group (n =10; mean age 22.3 +/- 1.76 years). On the first day of the study, 3 mL of venous blood samples were collected into ethylenediaminetetraacetic acid tubes by a specialist to measure N-Terminal Pro-B-Type Natriuretic Peptide for cardiac damage and Creatine Kinase (CK) for muscle damage. On the second day of the study, the Yo-Yo Intermittent Recovery Test Level-1 (YIRT1) was conducted to assess the players' maximum oxygen consumption (VO(2)max) and maximum aerobic speed (MAS) values. After six weeks of endurance training, the procedures above were repeated. A statistically significant difference between the pre-test and post-test data of CK, NT-ProBNP and VO(2)max in the experimental group (p <0.01) was observed. No statistically significant difference was observed between the pre and post-test data in the control group (p> 0.05). Additionally, based on the analysis of variance repeated measured analysis, a statistically significant difference was observed between the experimental and control groups regarding the time and time x group variables for NT-ProBNP and VO(2)max parameters (p < 0.01). However, no statistically significant difference was observed for CK levels (p> 0.05). A statistically significant difference was observed in CK levels in terms of the group variable (p < 0.01), while no statistically significant difference was observed in NTProBNP and VO(2)max levels (p> 0.05). Ultimately, our study revealed that endurance training positively affects heart and muscle damage.
Short-term Administration of Naringin Improves Renal Function in Renal Ischemia-reperfusion by Increasing Aquaporin-1 and Aquaporin-2 Levels
(Bentham Science Publ Ltd, 2024) Demir, Zübeyde; Acar, Gözde; Daşdelen, Derviş; Moğulkoç, Rasim; Baltacı, Abdulkerim Kasım
Background: Since renal ischemia-reperfusion (I/R) can lead to a serious health problem, aquaporins have important roles in preventing negative changes in electrolyte-water balance. This study aimed to determine the effect of naringin treatment on renal function and AQP1 and AQP2 levels in the kidney cortex and medulla tissues in experimental renal I/R in rats. Materials and Methods: The study was carried out on 40 male Wistar-type rats, 8-12 weeks old. Experimental groups were formed as follows: 1) Control, 2) Sham+vehicle, 3) Renal (I/R)+vehicle, 4) Renal I/R+ Naringin (50 mg/kg/day) (3 days of administration), and 5) Renal I/R+ Naringin (100 mg/kg/day) (3 days supplementation) group. First, the left kidney was removed by nephrectomy under general anesthesia, and then the right kidney was subjected to 45 minutes of ischemia and then 72 hours of reperfusion. Naringin was given to the experimental animals by an intraperitoneal route at the beginning of the reperfusion, after 24 and 48 hours. At the end of the experiments, first of all, blood samples were taken from the heart in animals under general anesthesia, and then the animals were killed by cervical dislocation, and kidney tissue samples were taken. Osmolarity in plasma and urine and plasma creatinine levels were evaluated. AQP1 and AQP2 levels were analyzed in the kidney cortex and medulla tissues by ELISA and PCR methods. Results: In kidney tissues, I/R led to a decrease in plasma and urinary osmolarity, AQP1 and AQP2 levels in the cortex and medulla, and an increase in urea and creatinine levels (p < 0.001). However, naringin supplementation corrected the deterioration to a certain extent. Conclusion The results of the study show that naringin supplementation at different doses, such as 50 or 100 mg/kg, may have protective effects on the deterioration of renal function caused by unilateral nephrectomy and I/R in rats.
Comparison of the effectiveness of thymoquinone, St. John Wort Oil and Silver Sulfadiazine in experimental burn wounds
(Oxford Univ Press, 2024) Canbaz, Yasin; Karakol, Percin; Erten, Remzi; Mercantepe, Tolga; Alp, Hamit Hakan; Canbaz, Esra Turan; Turel, Idris; Rağbetli, Murat Çetin
We aimed to compare the effectiveness of thymoquinone (TQ), the most important bioactive component of black cumin, St. John wort (SJW) oil, a traditional medicinal plant used in burns, and silver sulfadiazine (AgSD), a well-known antiinflammatory agent used in modern medicine, in an experimental burn rat-model. Sixty-three Wistar-Albino rats were randomly divided into 9 groups (n = 7). TQ and SJW were administered topically and systemically but AgSD was applied topically. Epithelialization, inflammatory cell response, granulation tissue, vascularization, and fibrosis were evaluated. Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), vitamin E, 8-hydroxy-deoxyguanosine (8-OHdG), coenzyme Q10 (CoQ10) were analyzed in serum. Topical TQ accelerated the epithelialization, enabled granulation, vascularization, and fibrosis in wounds (P = .001). Topical and systemic TQ increased Vitamin E levels (P = .003) but reduced TOS and 8-OHdG levels (P = .001). Topical SJW reduced granulation and vascularization. Topical and systemic SJW decreased TOS, MDA, and 8-OHdG levels (P = .001) but increased TAS (P = .001) and Vitamin E levels (P = .003). Topical AgSD reduced TOS, 8-OHdG, and MDA levels (P = .001). Topical and systemic TQ demonstrated significant advantages in accelerating the wound healing process while also enhancing antioxidant defenses and reducing oxidative damage. SJW oil, particularly in topical application, improved epithelialization, and antioxidant status but showed less efficacy in systemic use. AgSD, while effective in reducing oxidative stress, was less successful in promoting wound healing and appeared to delay granulation and fibrosis. Thymoquinone offers superior protective and healing benefits, SJW is effective locally but less so systemically, and AgSD should be used cautiously, potentially combined with antioxidants to mitigate its negative impact on wound healing.
PI3K AND MTOR IMMUNOREACTIVITY IN TESTICULAR TISSUE IN EXPERIMENTAL ALCOHOL ADDICTION MODEL
(2024) Öztürk, Osman; Okan, Aslı; Kurt, Mustafa; Eroğlu, Ece; Yılmaz, Seher; Uğuz, Abdulhadi Cihangir; Ocak, Mert
Alcohol use disorder has negative effects on the reproductive system through the development of oxidative stress and its genotoxic effects on DNA integrity. Acute and chronic alcohol use adversely affects the male reproductive system. Phosphatidylinositol-3-kinase (PI3K) and mammalian target of rapamycin (mTOR) levels, which are involved in oxidative stress, may be important in the reproductive system in alcohol use disorder. PI3K and mTOR immunoreactivities were evaluated in testicular tissue in an experimental acute and chronic alcohol intake model in male rats. Rats were divided into 3 groups as control male (n=7), acute male (n=7) and chronic male model (n=7). Histopathological analysis of testicular tissues taken from the experimental groups was performed by hematoxylin-eosin (H&E) staining. Then, testicular tissues of the experimental groups were dissected and PI3K and mTOR expressions were determined by immunohistochemistry method. According to the H&E staining results, when the experimental groups were compared with the control group; It was observed that spermatozoa were less or absent in acute and chronic groups. mTOR and PI3K expressions were significantly increased in testicular tissues belonging to chronic and acute alcohol model groups. In the chronic alcohol model, both mTOR and PI3K expressions were significantly increased compared to the acute and control groups. Our research reveal that PI3K and mTOR molecules, which are involved in oxidative stress in acute and chronic alcohol intake, may be associated with damage to the reproductive system. PI3K and mTOR proteins, can be targeted at the point of treatment against alcohol-induced reproductive damage.
Farklı Dozlarda Uygulanan Streptozotosinin Sıçan Karaciğerine Etkilerinin Stereolojik Metotlarla İncelenmesi
(2024) Yıldız, Saadet; Rağbetli, Murat Çetin; Tülüce, Yasin
Diabetes Mellitus, vücudun insülin üretememesinden veya insülin etkisine karşı dirençten veya her ikisinden kaynaklanan yüksek kan şekeri seviyeleri ile karakterize edilen bir grup kronik metabolik rahatsızlıktır. İnsülin, vücut dokuları ve organları tarafından glikoz üretimini ve kullanımını düzenleyen ana hormondur. Diyabet, karaciğer de dahil olmak üzere vücuttaki tüm sistemleri etkiler. Streptozotosin, pankreas ?-hücre yıkımı gerçekleştiren bir antibiyotiktir ve deneysel diyabet oluşturmak için yaygın olarak kullanılır. Bu çalışmada, sıçan karaciğerinde sterptozotosin kaynaklı diyabetin neden olduğu morfometrik değişikliklerin hacim ve hücre sayılarının stereolojik yöntemler kullanılarak hesaplanması amaçlandı. Çalışmada erişkin 20 adet erkek sıçan kullanıldı. Rastgele dört grup oluşturuldu. Tüm grupların kan glikoz değerleri ölçüldü. Streptozotosinin farklı dozları (55 mg/kg ve 65 mg/kg) verilerek diyabet indüklendi. 48 saat sonra tekrar kan glikoz değerleri ölçüldü. Dört hafta sonra kontrol ve denek gruplarına ait tüm sıçanların anestezi altında karaciğer dokuları çıkarılarak %10’luk formaldehitte tespit edildi. Tespit edilen karaciğer dokuları fiziksel fraksiyonlama ile eşit parçalara ayrıldı. Dehidratasyon aşamalarından geçerek parafinde sabitlendi. Histolojik kesitler hazırlandı. Hematoksilen-Eozin ile boyandı ve ışık mikroskobunda incelenerek fotoğraflandı. Cavalieri Prensibi ile karaciğerin total hacmi, disektör yönteminin tarafsız sayım çerçevesi ile hepatosit sayıları hesaplandı. Diyabete bağlı gruplarda, kontrol grubuna göre karaciğer hacminde ve hücre sayısındaki artış anlamlıydı (p<0.05). Sonuç olarak, streptozotosin enjeksiyonu ve bunun sonucunda ortaya çıkan diyabet, karaciğer hacminde ve hepatosit sayılarında artışa yol açmaktadır.
Effects of diclofenac sodium exposure during pregnancy on COX-2 expression in the ductus epididymis and ductus deferens of the offspring rat
(Ondokuz Mayis Universitesi, 2024) Kuşcu, Oğuzhan; Altındağ, Fikret; Aslan, Rahmi; Rağbetli, Murat Çetin
The present study aimed to investigate the effects of diclofenac sodium application during pregnancy on COX-2 expression in the ductus epididymis (DE) and ductus deferens (DD) of the offspring rat. 8 Eight adult Wistar Albino non-pregnant female rats and two breeding male rats were utilized. Rats determined to be pregnant were separated into two categories as sham (S) and diclofenac sodium (DS). It was injected into the S group with 1 ml/kg/day saline for 10 days, and it was injected into the DS group with 6.1 mg/kg/day DS. 7 male offspring were used in each group. DS decreased the serum testosterone levels and incresed the number of mast. DS induced fibrotic thickening. Offspring of S group had moderate COX-2 in the caput epididymis (CPE) and severe COX-2 in the cauda epididymis (CE), while those in the DS group had weak COX-2 in the CPE and moderate COX-2 in the CE. There was no COX-2 expression in the vas deferens and corpus epididymis (COE) of the offspring rats in the S and DS groups. TNF-α expression was not observed in the S and DS groups. It was observed that moderate IL-10 expression in the CPE and CE, and weak IL-10 expression in the vas deferens and COE in the S and DS groups. The findings of our study reveal that DS administration during pregnancy can cause the downregulation of COX-2 expression in the DE and DD of offspring rats and therefore may have adverse effects on the male reproductive system.
Water-soluble Pillar[5]arene-based drug candidates for lung and breast cancer
(Taylor and Francis Ltd., 2024) Duran, Tuğce; Karaselek, Mehmet Ali; Küççüktürk, Serkan; Kursunlu, Ahmed Nuri; Özmen, Mustafa
The objective of research was to examine the likely anticancer effectiveness of distinct pillar[5] arene derivatives, ws-penta-P[5] and ws-deca-P[5], on breast and lung cancer cell lines in vitro. To achieve this goal, breast cancer (MCF7) cells, lung cancer (A549) cells, healthy cells (HEK293) were utilized. The IC50 dose of ws-penta-P[5] and ws-deca-P[5] was determined using the MTT method. Both treatment (pillar[5] arene applied) and control (pillar[5] arene not applied) groups were established for all three cell lines. Real-time polymerase chain reaction (qPCR) was used to evaluate changes in gene expression following pillar[5] arene treatment. Flow cytometry analysis was used to determine apoptosis and cell cycle arrest. The treatment group and control group results were compared after the study. The results revealed that in both cell lines treated with ws-deca-P[5], proapoptotic gene expressions were upregulated, while antiapoptotic gene expressions and caspase activation gene expressions were down-regulated. The flow cytometry apoptosis and cell cycle analysis in treatment group compared to the control, it was observed that the apoptosis rate increased in the ws-deca-P[5] and ws-deca-P[5] were shown to cause G0/G1 phase arrest in both cell groups. Results from our study that pillar[5] arene derivatives had the potential for treating breast and lung cancer, and more research is required in this area. Communicated by Ramaswamy H. Sarma.
Exploring the combined anti-cancer effects of sodium butyrate and celastrol in glioblastoma cell lines: a novel therapeutic approach
(Springer, 2024) Kartal, Bahar; Denizler-Ebiri, Farika Nur; Güven, Mustafa; Taşpınar, Filiz; Canpınar, Hande; Çetin, Sedat; Karaduman, Tuğçe; Küççüktürk, Serkan
Glioblastoma, a highly aggressive and lethal brain cancer, lacks effective treatment options and has a poor prognosis. In our study, we explored the potential anti-cancer effects of sodium butyrate (SB) and celastrol (CEL) in two glioblastoma cell lines. SB, a histone deacetylase inhibitor, and CEL, derived from the tripterygium wilfordii plant, act as mTOR and proteasome inhibitors. Both can cross the blood–brain barrier, and they exhibit chemo- and radiosensitive properties in various cancer models. GB cell lines LN-405 and T98G were treated with SB and CEL. Cell viability was assessed by MTT assay and IC50 values were obtained. Gene expression of DNA repair, apoptosis, and autophagy-related genes was analyzed by RT-PCR. Cell cycle distribution was determined using flow cytometry. Viability assays using MTT assay revealed IC50 values of 26 mM and 22.7 mM for SB and 6.77 μM, and 9.11 μM for CEL in LN-405 and T98G cells, respectively. Furthermore, we examined the expression levels of DNA repair genes (MGMT, MLH-1, MSH-2, MSH-6), apoptosis genes (caspase-3, caspase-8, caspase-9), and an autophagy gene (ATG-6) using real-time polymerase chain reaction. Additionally, flow cytometry analysis revealed alterations in cell cycle distribution following treatment with SB, CEL and their combination. These findings indicate that SB and CEL may act through multiple mechanisms, including DNA repair inhibition, apoptosis induction, and autophagy modulation, to exert their anti-cancer effects in glioblastoma cells. This is the first study providing novel insights into the potential therapeutic effects of SB and CEL in glioblastoma
Gender-related variation expressions of neuroplastin TRAF6, GluA1, GABA(A) receptor, and PMCA in cortex, hippocampus, and brainstem in an experimental epilepsy model
(John Wiley and Sons Inc, 2024) Doğanyiğit, Züleyha; Okan, Aslı; Yılmaz, Seher; Uğuz, Abdülhadi Cihangir; Akyüz, Enes
Epileptic seizures are seen as a result of changing excitability balance depending on the deterioration in synaptic plasticity in the brain. Neuroplastin, and its related molecules which are known to play a role in synaptic plasticity, neurotransmitter activities that provide balance of excitability and, different neurological diseases, have not been studied before in epilepsy. In this study, a total of 34 Sprague–Dawley male and female rats, 2 months old, weighing 250–300 g were used. The epilepsy model in rats was made via pentylenetetrazole (PTZ). After the completion of the experimental procedure, the brain tissue of the rats were taken and the histopathological changes in the hippocampus and cortex parts and the brain stem were investigated, as well as the immunoreactivity of the proteins related to the immunohistochemical methods. As a result of the histopathological evaluation, it was determined that neuron degeneration and the number of dilated blood vessels in the hippocampus, frontal cortex, and brain stem were higher in the PTZ status epilepticus (SE) groups than in the control groups. It was observed that neuroplastin and related proteins TNF receptor-associated factor 6 (TRAF6), Gamma amino butyric acid type A receptors [(GABA(A)], and plasma membrane Ca2+ ATPase (PMCA) protein immunoreactivity levels increased especially in the male hippocampus, and only AMPA receptor subunit type 1 (GluA1) immunoreactivity decreased, unlike other proteins. We believe this may be caused by a problem in the mechanisms regulating the interaction of neuroplastin and GluA1 and may cause problems in synaptic plasticity in the experimental epilepsy model. It may be useful to elucidate this mechanism and target GluA1 when determining treatment strategies.
Comparative cross-sectional study on sexual function of couples during pregnancy: Assisted reproductive techniques versus spontaneous conceptions
(Women's Health and Action Research, 2024) Dülger, Özlem; Dinmez, Sinem; Torun, Sebahat D.; Osmanoğlu, Usame Ömer
This study aims to compare the sexual functions of couples undergoing assisted reproductive techniques (ART) with those conceiving spontaneously during pregnancy. A total of 102 couples participated in this cross-sectional study, with 68 couples in the spontaneous conception group and 34 couples in the ART group. Data collection was conducted face-to-face in the antenatal clinic using separate “Descriptive Information Form” for women and men, “Female Sexual Function Index (FSFI)” for women, and “Libido Scoring System (LSS)” for men. Descriptive statistical methods, Chi-square and Fisher exact tests, t-test, and Pearson correlation test were used for data analysis. It was observed that the mean FSFI total scores of women who conceived spontaneously and through ART during pregnancy were at a good level (≥30), while the mean LSS scores of their partners were at a moderate level (5-7). No statistically significant distinction existed among the groups. Further research is advisable by adjusting the sample selection criteria, such as gravida, duration of ART treatment, and gestational age.
The effects of Garcinia kola and curcumin on the dorsal root ganglion of the diabetic rat after peripheral nerve transection injury
(Elsevier B.V., 2024) Aktaş, Abit; Yiğit, Funda; Delibaş, Burcu; Kaplan, Arife Ahsen; Hamour, Hala Mahgoub; Kaya, Ayşenur
Objective: To test the protective effects of Garcinia kola and curcumin on the ganglion tissues of diabetic rats following the use of autologous vein graft in peripheral nerve transection injury. Methods: The sciatic nerve on the right side was transected, and anastomosis was performed between the proximal and distal ends using an autologous vein graft. Curcumin and Garcinia kola seed extract were administered daily by oral gavage. The ganglion tissues were harvested after a 90-day waiting period. Sensory neurons in the dorsal root ganglion at the L4 and L5 levels were used for stereological evaluations. Mean sensory neuron numbers were analyzed using a stereological technique. The size of the light and dark neurons was also estimated, and ultrastructural and immunohistochemical evaluations were performed. Results: A statistically significant difference in sensory neuron numbers was observed between the groups with and without Garcinia kola and curcumin applications. The immunohistochemical results showed that the s-100 protein is expressed selectively between cell types. Conclusion: The results of this study show that curcumin and Garicinia kola prevented sensory neuron loss in diabetic rats following transection injury to the sciatic nerve.
Evaluation of in vitro antimicrobial activity of Epigallocatechin gallate (EGCG) and green tea (Camellia sinensis) oil on various pathogens.
(Genel Tıp Dergisi, 29.02.2024) Koksoy, Hale; Ragbetli, Cennet
ABSTRACT Aim: Epigallocatechin gallate (EGCG), the polyphenolic component of Camellia sinensis catechins, and Camellia sinensis extract have broad antimicrobial activity. This study aimed to investigate the invitro antibacterial and antifungal activity of the Epigallocatechin gallate and Camellia sinensis extract. Method: The present study tested the antibacterial and antifungal activity of Epigallocatechin gallate and Camellia sinensis extract against some gram-negative, gram-positive, and fungal isolates of American Type Culture Collection (ATCC). The minimum inhibitory concentration (MIC) of Epigallocatechin gallate (EGCG) and Camellia sinensis extract were determined for each test microorganism. In general, EGCG and Camellia sinensis extract results were found compatible. Epigallocatechin gallate and Camellia sinensis extract exhibited antibacterial and antifungal activity against all test organisms. MIC was determined visually after 16-20 hours of incubation at 37°C according to broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI). Result: The Camellia sinensis extract and EGCG MIC for gram-positive bacteria was found lower than the MIC for gram-negative bacteria and the Camellia sinensis extract MIC for C. albicans was higher than the EGCG MIC. Conclusions: However, EGCG was more effective on C. albicans isolate than Camellia sinensis extract. Camellia sinensis extract Epigallocatechin gallate and Camellia sinensis extract may be a promising source of antibacterial and antifungal for further studies. Keywords: Epigallocatechin gallate (EGCG), Green tea, Camellia sinensis, Antibacterial effect, Antifungal effect.
Investigation of hip extension and flexion muscle strength in female weightlifting athletes
(2023) Işık, Bülent; Erdağı, Kenan
Objective: It was aimed to investigate hip flexion and extension muscle strength of female weightlifters athletes participating in national and international weightlifting championships. Methods: Hip extension, hip flexion strength measurements, weightlifting performances and demographic data of female athletes participating in international weightlifting championships (n= 15) and female athletes participating in national weightlifting championships (n= 15) included in our study were determined. Hip flexion/extension muscle strength measurements of the athletes were performed with a Lafayette hand-held dynamometer. Results: It was observed that hip flexion and extension muscle strength values of female weightlifters who do weightlifting at international level values were higher than the values of female weightlifters who do weightlifting at the national level (p < .05). There was a statistically significant difference between right-left hip-flexion values in female weightlifting athletes who do elite weightlifting sports (p < .05). Conclusion: It was determined that hip extension-flexion muscle strength of athletes participating in international championships were higher than hip extension-flexion muscle strength values of athletes participating in national championships. Considering that asymmetrical developments may cause sports injuries, it is thought that it is important to evaluate the asymmetrical development of hip muscle groups in certain periods.
The effect of curcumin on penile fibrotic plaque in rats with experimental peyronie’s disease
(2023) Ulubay, Mahmut; Kaya, Ayşenur; Kaplan, Süleyman; Kaplan, Arife Ahsen; Tüfekçi, Kıymet Kübra; Akdeniz, Ekrem
Objective: No effective medical approach for the treatment of Peyronie’s disease (PD) has to date been described. This study was intended to evaluate the antifibrotic, antioxidant, and anti-inflammatory effects of curcumin on fibrotic tissue in the tunica albuginea (TA) in a rat model of PD. Materials and Methods: Twenty-four male Sprague Dawley rats aged 10 months were randomized into three groups (n = 8 in each). No PD model was induced in the control group. The PD+saline (PD+Ps) group received fibrin injection, followed two weeks later by saline administration by oral gavage for 14 days. The PD+Curcumin (PD+Cur) group received fibrin injection into the TA followed two weeks later by curcumin administration by oral gavage for 14 days. At the end of the experiment, fibrotic activity was evaluated using stereological and histopathological methods. Transforming growth factor-?1 (TGF-?1), one of the most fibrogenic cytokines, was evaluated using immunohistochemistry with an anti-TGF-?1 rabbit monoclonal antibody. Results: Stereological analysis revealed significantly greater Peyronie-like plaque areas in the TA in the PD+Ps group than in the control and PD+Cur groups (p<0.0001). No significant difference was observed between the control and PD+Cur groups (p=0.35). The PD+Ps group exhibited strong TGF?1 immunoreactivity with increased expression in the collagenous connective tissues and fibroblasts around the TA. Conclusion: Curcumin reduced fibrotic tissue in the TA and may represent a novel therapeutic option in the treatment of PD.

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