Kamil Özdağ Fen Fakültesi, Biyoloji Bölümü, Bildiri & Sunum Koleksiyonu
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Öğe The effects of Salvia absconditiflora water extract on HepG2 liver cell line(Wiley, 2014) Kartal, D. Irtem; Sadi, Gökhan; Guray, T. N.…Öğe Effect of the bioactive components of Salvia absconditiflora on gene expressions of HepG2 cell line(Wiley-Blackwell, 2015) Kartal, D. İrtem; Sadi, Gökhan; Güray, N. T.The liver is the principal organ of metabolism in the body. It hasspecialized tissues consisting of mostly hepatocytes that regulatesa wide variety of biochemical reactions. Liver disease causedabout 10% of all human population. There are around 900 spe-cies of Salvia species, 95 of which are represented in Turkey con-sumed as herbal tea. They have been used since ancient times formore than 60 different diseases such as cancer, aging and etc.Öğe Changes in hepatic insulin signaling and inflammatory responses in streptozotocin induced diabetes: Effects of resveratrol(Wiley-Blackwell, 2015) Sadi, Gökhan; Pektaş, M. B.; Koca, H. B.; Tosun, M.; Koca, T.40th Congress of the Federation-of-European-Biochemical-Societies (FEBS) - The Biochemical Basis of Life Location: Berlin, GERMANY Date: JUL 04-09, 2015Öğe High fructose diet up-regulates insulin and its down-stream signaling components in abdominal ommentum tissues: effects of resveratrol(Wiley-Blackwell, 2015) Yalçın, H. A.; Sadi, Gökhan; Pektaş, M. B.; Akar, F.40th Congress of the Federation-of-European-Biochemical-Societies (FEBS) - The Biochemical Basis of Life Location: Berlin, Germany Date: JUL 04-09, 2015Öğe Effect of Acorus calamus and Apium graveolens extracts on Egfr and Erbb2 in LNCaP cells(Wiley, 2018) Koca, H. B.; Sadi, Gökhan; Köken, T.; Koca, T.Prostate cancer is one of the most commonly diagnosed cancers in men of developed Western countries. Several treatments are available for treatment of prostate cancer. These include surgery, radiation, radioactive implants and hormonal therapy. However, the treatment often impacts the quality of life due to side‑effects or complications. Thus, numerous investigators have focused on discovering novel drugs or treatments. Among all the agents tested, natural products derived from medicinal plants are among the most favorable. Migration and invasion of cancer cells is regulated by multiple pathways that employ various growth factor and their receptors, integrins and cytoskeletal elements. A key role is played by the EGF receptor (EGFR), which, following interaction with the integrin a6b4, promotes cell migration through activation of PI3K and other downstream pathways. The androgen receptor can be activated indirectly by growth factor receptors, mainly the transmembrane tyrosine kinases EGFR and ERBB2, members of the epidermal growth factor receptor (EGFR) family, which are activated inappropriately in many human cancer types. It has been reported that the EGF receptors EGFR (HER1) and ERBB2 (HER2 / neu) are overexpressed in metastatic prostate tumors. In this study, we investigated the effect of A. Calamus and A. Graveolens extracts on Egfr and Erbb2 on the human prostatic carcinoma cell line LNCaP. LNCaP cells were treated with increasing concentrations of an ethanolic extract of A. graveolens ranging from 1000 to 3000 µg/ml, A. Calamus 250 to 750 µg/ml and viability was determined after 24 and 48 h using the XTT cell proliferation assay. The levels of EGFR and ERBB2 were analyzed. Finally, quantitative gene expression analysis of EGFR and ERBB2, was performed using realtime reverse transcription– polymerase chain reaction. As a result, we observed that A. calamus and A. graveolens extracts affected EGFR and ERBB2 levels in LNCaP cellsÖğe Status of aquatic plant tissue culture in Turkey(Current Biology Ltd, 2011) Khawar, Khalid Mahmood; Aasim, Muhammad; Öztürk, Meryem; Bakhsh, Allah; Özcan, Sebahattin; Atar, Hasan HüseyinMany fascinating and attractive plants are grown world wide in aquariums for beauty, to maintain the quality of water besides providing food, shade, shelter and breeding places for many life forms including fish. Global trade has enabled people in Turkey to benefit from the unprecedented movement and establishment of new but locally unknown ornamental aquarium plant species. Local demand for aquatic plants has shown a steady increase in the past few years. In the absence of established propagation systems, local traders import aquatic ornamental plants illegally to meet the high demand of local people. If proper checks and measures are not taken, this may result in pollution of local aquatic ecosystems in the future. Plant tissue culture has made an appreciable contribution to micro-propagation of a wide range of ornamental, horticultural and other plants. However, tissue culture studies in aquatic plants are not impressive, with few published reports. The use of plant tissue culture techniques for the propagation of aquatic plants can help in reaping many benefits and advantages, including preventing threats to environment. This paper reviews efforts to introduce aquatic plant tissue culture along with its present status in TurkeyÖğe In vitro rooting without exogenous auxins and acclimatization of Fritillaria species of Turkey(Current Biology Ltd, 2011) Gürlek, Derya; Aasim, Muhammad; Özcan, SebahattinTurkey has great genetic diversity of bulbous plants (geophytes) with beautiful flowers, used as ornamental plants, and also containing alkaloids, with considerable potential for their use in the perfume and pharmaceutical industries. Fritillaria is one of the important geophytes of Turkey, which has the maximum number (36) of species of Fritillaria in the world. Rooting and acclimatization are important steps in the successful micropropagation of bulbous plants, and can be achieved by applying exogenous auxins as common practice under in vitro conditions. This study reports in vitro rooting and acclimatization of Fritillaria imperialis and F. persica (Siverek and Mersin) — genus Fritillaria of the Liliaceaea family — without exogenous application of auxins in the culture medium. Different concentrations of salts (KCl, NaCl and CaCl2); different gelling agents (agar, gelrite) and use of gelling agent + temperature were tested for in vitro rooting followed by acclimatization under greenhouse conditions. NaCl at 10 g/l followed by transfer to greenhouse conditions after 15 days, were found to be more suitable for acclimatization.Öğe Past, present and future of tissue culture and genetic transformation research on cowpea (Vigna unguiculata L.)(Current Biology Ltd, 2011) Aasim, Muhammad; Bakhsh, Allah; Khawar, Khalid Mehmood; Özcan, SebahattinCowpea (Vigna unguiculataL.) is one of the most importantdrought-tolerant summer food legume crop largely cultivated ontens of millions of small farms in the drier zones of Africa withlimited yield due to various biotic and abiotic stresses. It is alsogrown in some Asian, South European, Central and South Ameri-can countries both as food and forage crops. Cowpea can play animportant role in supplementing rural population diet due to itslow cost high protein, high calories, minerals and vitamins. Worlddemand for legume feed protein is increasing gradually due toincreasing demand for animal food products. Therefore, there is adire need to develop new varieties combining traditional and mod-ern biotechnological techniques to meet these future challenges.Reports concerning micropropagation and tissue culture studies ofcowpea started during early 1980s with moderate success in 1990sowing to recalcitrant nature of plant. However, during 2000s manyresearchers developed new protocols to overcome the problemof recalcitrance and consequently micropropagation. This reportreviews past and present tissue culture and genetic transformationresearch on cowpea and also focus on future prospects of cowpearesearch using modern biotechnological techniquesÖğe RuBisCo small subunit as strong green tissue specific promoter(Current Biology Ltd, 2011) Baloch, Faheem Shahzad; Bakhsh, Allah; Aasim, MuhammadTranscriptomics has the potential to rapidly increase our knowl-edge of spatial and temporal gene expression and lead to newpromoters for research and development. The availability of abroad-spectrum promoters having the ability to regulate the tem-poral and spatial expression patterns of the transgene can increasethe successful application of transgenic technology and to addresslegitimate concerns raised about the safety and containment oftransgenic plants. Compared with the temporal-specific or spatial-specific expression of the toxin, constitutive expression of foreignproteins in transgenic plants may cause adverse effects. Consti-tutive overexpression of transgenes that interfere with normalprocesses in a plant underscore the need for refinement of trans-gene expression. The development of tissue-specific promoters todrive transgene expression has helped to fulfill that need. There-fore, it is desirable to use expression-specific promoters which onlyexpress the foreign gene in specific plant tissues or organs. Thisstudy highlights the uses and benefits reaped by researchers byusing green tissue specific promoter (RuBisCo small subunit) in dif-ferent crops and systems and establishes a broad range of tissuespecific promoters. Such plant promoters that are activated pre-cisely when and where they are needed would be ideal for geneticengineering strategies.Öğe Topraktan Ksilanaz Pozitif Mikroorganizmaların İzolasyonu(Yayıncı Yok, 2013) Gümüşkaya, Nermin; Tofur, Arife; Kulak, Hürü; Gönek, Serap; Kocabaş, AytaçGünümüzde mikroorganizmalar ve mikroorganizma kaynaklı enzimler hemen hemen bütün endüstri dallarında görev almaktadır ve gittikçe kullanım alanları genişlemektedir. Kullanım alanlarının yaygınlaşmasını ve kullanım miktarlarının artmasının sebeplerinden en önemlisi her geçen gün yapılan araştırmaların artması, yeni özelliklere sahip enzimlerin keşfedilmesidir. Bunlara ek olarak, hali hazırda kullanılan enzimlerin optimizasyon çalışmaları da ayrı bir odak noktası olmaktadır. Enzimler ya üreten mikroorganizmanın direkt olarak ya da enzimin kendisinin kullanılması şeklinde endüstride görev yapmaktadır.Öğe Karaman İlinde Yetiştirilen Elma Çeşitlerinin Antimikrobiyal Aktivitelerinin Belirlenmesi(Yayıncı Yok, 2013) Başkaya, Yasemin; Güder, Sevgi; Karaman, Mehmet Akif; Kocabaş, Aytaç; Sutay Kocabaş, DidemGünümüzde çeşitli mikrobiyal hastalıklara karşı bilinçsizce antibiyotiklerin kullanılması sonucunda mikroorganizmalarda antibiyotik dirençliliği gelişmekte ve bu nedenle hastalıkların tedavisinde mevcut antibiyotiklerin kullanılabilirliği azalmaktadır. Bu olumsuz gelişme nedeniyle, yeni ve etkin antibiyotiklerin bulunması gerekmektedir. Ayrıca, gıda güvenliği de önemi hızla artan bir konudur. Son yıllarda gıda kaynaklı enfeksiyonlara bağlı hasta sayısı giderek artmaktadır. Araştırmalara göre, sanayileşmiş ülkelerde gıda kaynaklı hasta sayısı toplam nüfusun %30'u kadardır. Bu noktada, doğal kaynaklı yeni antibiyotiklerin geliştirilmesi önem kazanmaktadır. Bilindiği üzere, sentetik olarak üretilen ürünler çok daha fazla yan etkiyi de beraberinde getirmektedir. Bu nedenle günümüzde bitkisel kaynaklı maddeler doğal olmaları ve doğada bozunabilirliklerinin yüksek olması nedeniyle araştırmacıların ilgisini çekmektedir. Bu noktada araştırmalar çoğunlukla baharatlar ve günlük kullanılan bitkiler üzerinde yoğunlaşmıştır. Bitkilerden elde edilen uçucu yağlar ve diğer özütler, antimikrobiyal özellikleri sayesinde hem hastalıkların tedavisinde hem de gıda ürünlerinin korunmasında kullanılma potansiyeline sahiptirler.Öğe Hot Filament Chemical Vapor Deposition of Polymeric Tertiary Amine Containing Nanocoatings For Antibacterial Textile Applications(Yayıncı Yok, 2014) Sevgili, Emine; Mutlu, Burak; Kocabaş, Aytaç; Sadi, Gökhan; Yıldız, H.Bekir; Martı, Mustafa Esen; Karaman, MustafaIn this study, poly(diethyl aminoethyl methacrylate) (PDEAEMA) thin films were deposited by hot filament chamical vapor deposition (HFCVD) method on cotton fabric surfaces to prepare antibacterial textile materials. Use of an initiator tert-butyl peroxide (TBPO) during the depositions allowed coatings at low filament temperatures. Deposition rate was measured as 25 nm/min. FTIR analysis of the coatings confirmed the high retention of the tertiary amine functional group that is required for the antibacterial activity. SEM images showed conformal coatings around individual fibers of the cotton fabric.Öğe Aspergillus terreus Ksilanaz Üretim Koşullarının Batık Kültürde İstatistiki Metodlarla Optimizasyonu(Yayıncı Yok, 2014) Gümüştaş, Nermin; Kocabaş, AytaçEndüstrinin neredeyse her alanında kullanılan enzimler genellikle mikroorganizma kaynaklıdırlar.Bunun sebebi mikroorganizma kökenli enzimlerin, bitkisel veya hayvansal kaynaklı enzimlere göre katalitik aktivitelerinin çok yüksek olması, daha ucuz olmaları, ekstremkoşullarda aktivite gösterebilmeleri ve çok miktarda üretilebilmeleridir. Enzimler; gıda, tekstil, ekmek, deterjan, içecek üretimi ve saflaştırılması, yenilenebilir enerji kaynaklan ve kimya endüstrisi gibi yaygın kullanım alanlanbelirlenmiştir. Optimizasyon çalışmalan hem organizmanın hem de üretilecek ksilanaz enziminin endüstriyelöneme sahip olması nedeniyle organizmanın ticari üretim aşamasında enzim üretiminin iyileştirilmesi açısından önem arz etmektedir.
