Biochemical and molecular characterization of bread wheat genotypes under drought stress: Implications for antioxidant defense mechanisms and genomic analysis
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
University of Ljubljana
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Plants face abiotic stresses like drought, salinity, and high temperature, which adversely affect growth and induce physiological and metabolic changes. Drought is a complex stress controlled by many genes, requiring investigation through molecular markers and biochemical characterization in wheat genotypes. This study involved eight bread wheat cultivars and two controls: drought-tolerant ‘Gerek-79’ and drought-sensitive ‘Sultan-95’. These were grown for 40 days and then subjected to 10 days of drought stress. Antioxidants and antioxidant enzyme activities, which neutralize ROS, are key resistance mechanisms against oxidative stress. Levels of polyphenol oxidase (PPO), peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT) were measured. Plant responses to stress included changes in photosynthetic pigments, total proteins, hydrogen peroxide, lipid peroxidation (MDA), and proline levels. POD showed the highest change in enzyme activity, while PPO was least affected. Chlorophyll b levels increased under stress across all varieties. Notably, proline levels, an abiotic stress marker, significantly rose by the 10th day of drought. Additionally, wheat genotypes were analyzed using drought-related SSR markers (Xwmc 89, Xwmc 118, Xwmc 304, Xgwm 337). This allowed evaluation of the impact of molecular characterization on biochemical changes under drought stress. © 2024 University of Ljubljana. All rights reserved.
Açıklama
Anahtar Kelimeler
antioxidant enzymes, bread wheat, drought stress, hydrogen peroxide, lipid peroxidation, photosynthetic pigments, proline
Kaynak
Acta Agriculturae Slovenica
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
120
Sayı
3
