Evaluation of antioxidant activity of in vitro propagated medicinal ceratophyllum demersum l. extracts
Yükleniyor...
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wydawnictwo Akad Rolniczej W Lublinie
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25-1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (beta-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 mu g/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.
Açıklama
WOS:000429301600003
Anahtar Kelimeler
Shoot Regeneration, Antioxidant Component, Metal Chelating, Reducing Power
Kaynak
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
17
Sayı
1
Künye
Emsen, B., Doğan, M. (2018). Evaluation of antioxidant activity of in vitro propagated medicinal ceratophyllum demersum l. extracts. Acta Scientiarum Polonorum Hortorum Cultus, 17, 1, 23-33.
