In Vitro plant regeneration of Turkish sweet basil (Ocimum basilicum L.)
Yükleniyor...
Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pakistan Agricultural Scientists Forum
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Sweet Basil (Ocimum basilicum L.) is most important medicinal and aromatic plant used in aromatherapy, cosmetics, perfume and food products in fresh or dried form. The present study provides a reliable and reaptable in vitro plant regeneration protocol of cultivated sweet basil of Turkey. Basil seeds were surface sterilized with 2.5% NaOCl. Epicotyl, hypocotyl and shoot tip explants were isolated from 12-14 days old in vitro grown seedlings. The explants were cultured on MS medium containing 0.80-2.40 mg/L TDZ with or without 0.10 mg/L IBA alongwith 1.0 mg/L PVP and 3.0 g/L activated charcoal. Cent percent callus induction were recorded on all explants on all culture mediums. Shoot regeneration frequency of epicotyl, hypocotyl and shoot tip explant ranged 75.0-100, 25.0-83.33 and 66.67-100% respectively. Maximum number of shoots from epicotyl (3.22) and shoot tip (3.58) were scored on MS medium containing 2.40 mg/l TDZ-0.10 mg/l IBA. Whereas, hypocotyl explant induced maximum number of (5.17) shoots per explant on MS medium with 2.0 mg/l TDZ. Relatively shorter roots of less than 1.0 cm were recorded irrespective of explant type or culture medium. However, shoots were successfully rooted and plantlets were transferred to pots and acclimatized well in growth room conditions.
Açıklama
WOS:000347168800027
Anahtar Kelimeler
In Vitro, Epicotyl, Hypocotyl, Shoot Tip, Shoot Regeneration, Sweet Basil
Kaynak
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
24
Sayı
6
Künye
Ekmekçi, H., Aasim, M. (2014). In vitro plant regeneration of Turkish sweet basil (Ocimum basilicum L.). Journal of Animal and Plant Sciences 24 (6):1758-1765, December 2014.
