Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells

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dc.authorid0000-0002-6422-1203en_US
dc.contributor.authorAltay, Ahmet
dc.contributor.authorKartal, Deniz İrtem
dc.contributor.authorSadi, Gökhan
dc.contributor.authorGüray, Tulin
dc.contributor.authorYaprak, Ahmet Emre
dc.date.accessioned2019-12-06T21:16:08Z
dc.date.available2019-12-06T21:16:08Z
dc.date.issued2017
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.descriptionWOS:000408923400007en_US
dc.description.abstractPhase I-II detoxification and antioxidant enzymes are responsible for the detoxification and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in the metabolic activation of xenobiotics. The herb Salicornia freitagii (SF) (Amaranthaceae) is known for its anticancer, antioxidant, antidiabetic and antiinflammatory activities. In this study, we determined the bioactive phenolics in the SF methanol extract and investigated its antiproliferative potential in HT-29 human colon cancer cells. We also investigated the modulation of some phase I and II enzyme (CYP 1A1, 1A2, 2E1, GSTP1 and GPx) mRNA expression and enzymatic activities by the SF extract and its major bioactive phenolic compounds. LC/MS-MS analysis showed that the main phenolic compounds of the methanolic SF extract are vanillic acid (48 mu g/100g) and p-coumaric acid (10.8 mu g/100g). SF extract, vanillic acid and p-coumaric acid exhibited high antiproliferative activities in HT-29 cells, with IC50 values of 81.79 mu g/mL, 98.8 mu M and 221.6 mu M, respectively. The mRNA expression levels of CYP1A2 and CYP2E1 were decreased, while those of GSTP1 and GPx in HT-29 cells were increased after application of either the SF extract or vanillic acid. The SF extract by itself also increased the activities of GPx and GSTP1 enzymes 1.68- and 1.49-fold, respectively. Our data indicate that the SF extract and its major bioactive compound, vanillic acid, could exert a modulatory effect on the expression of enzymes that are involved in xenobiotic activation and detoxification pathways in the gastrointestinal tract. For this reason, SF can be considered as a natural source of chemopreventive agents.en_US
dc.description.sponsorshipNezahat Gokyigit Botanical Garden in Turkey [60.05.02]; METU in Turkey [BAP-08-11-DPT-2011K121010]en_US
dc.description.sponsorshipThe authors express their gratitude to Associate Professor Dr. Gulcin Celep (Gazi University) who kindly collected and provided the samples for the study. This work was financially supported by Nezahat Gokyigit Botanical Garden (Research Project No. 60.05.02) in Turkey and METU (Project No. BAP-08-11-DPT-2011K121010) in Turkey.en_US
dc.identifier.citationAltay, A., İrtem, K., Sadi, G., Güray, T., Yaprak, A. (2017). Modulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cells. Archives of Biological Sciences, 69, 3, 439-448.
dc.identifier.doi10.2298/ABS160825118A
dc.identifier.endpage448en_US
dc.identifier.issn0354-4664
dc.identifier.issn1821-4339
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85027376481
dc.identifier.scopusqualityQ3
dc.identifier.startpage439en_US
dc.identifier.urihttps://dx.doi.org/10.2298/ABS160825118A
dc.identifier.urihttps://hdl.handle.net/11492/2826
dc.identifier.volume69en_US
dc.identifier.wosWOS:000408923400007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorSadi, Gökhan
dc.language.isoen
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovicen_US
dc.relation.journalArchives Of Biological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGlasswortsen_US
dc.subjectCytotoxicityen_US
dc.subjectDrug Metabolismen_US
dc.subjectColon Canceren_US
dc.subjectPhase I-II Enzymesen_US
dc.titleModulation of mRNA expression and activities of xenobiotic metabolizing enzymes, CYP1A1, CYP1A2, CYP2E1, GPx and GSTP1 by the Salicornia freitagii extract in HT-29 human colon cancer cellsen_US
dc.typeArticle

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