Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production
Yükleniyor...
Dosyalar
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
E. coli plays a substantial role in recombinant protein production. Its importance increased with the discovery of recombinant DNA technology and the subsequent production of the first recombinant insulin in E. coli. E. coli is a widely used and cost-effective host to produce recombinant proteins. It is also noteworthy that a significant portion of the approved therapeutic proteins have been produced in this organism. Despite these advantages, it has some disadvantages, such as toxicity and lack of eukaryotic post-translational modifications that can lead to the production of misfolded, insoluble, or dysfunctional proteins. This study focused on the challenges and engineering approaches for improved expression and solubility in recombinant protein production in E. coli. In this context, solution strategies such as strain and vector selection, codon usage, mRNA stability, expression conditions, translocation to the periplasmic region and addition of fusion tags in E. coli were discussed.
Açıklama
PubMed ID: 38479588
Anahtar Kelimeler
Optimized Protein Production, Recombinant Protein, Solubility Of Protein
Kaynak
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
219
Sayı
Künye
İncir, İ., & Kaplan, Ö. (2024). Escherichia coli as a versatile cell factory: advances and challenges in recombinant protein production. Protein Expression and Purification. https://doi.org/10.1016/j.pep.2024.106463
