Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production

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dc.authorid0000-0003-4599-6908en_US
dc.contributor.authorİncir, İbrahim
dc.contributor.authorKaplan, Özlem
dc.date.accessioned2024-07-11T06:53:49Z
dc.date.available2024-07-11T06:53:49Z
dc.date.issued2024en_US
dc.departmentKMÜ, Kazım Karabekir Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.descriptionPubMed ID: 38479588en_US
dc.description.abstractE. coli plays a substantial role in recombinant protein production. Its importance increased with the discovery of recombinant DNA technology and the subsequent production of the first recombinant insulin in E. coli. E. coli is a widely used and cost-effective host to produce recombinant proteins. It is also noteworthy that a significant portion of the approved therapeutic proteins have been produced in this organism. Despite these advantages, it has some disadvantages, such as toxicity and lack of eukaryotic post-translational modifications that can lead to the production of misfolded, insoluble, or dysfunctional proteins. This study focused on the challenges and engineering approaches for improved expression and solubility in recombinant protein production in E. coli. In this context, solution strategies such as strain and vector selection, codon usage, mRNA stability, expression conditions, translocation to the periplasmic region and addition of fusion tags in E. coli were discussed.en_US
dc.identifier.citationİncir, İ., & Kaplan, Ö. (2024). Escherichia coli as a versatile cell factory: advances and challenges in recombinant protein production. Protein Expression and Purification. https://doi.org/10.1016/j.pep.2024.106463en_US
dc.identifier.doi10.1016/j.pep.2024.106463
dc.identifier.issn1046-5928
dc.identifier.pmid38479588
dc.identifier.scopus2-s2.0-85187644481
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1016/j.pep.2024.106463
dc.identifier.urihttps://hdl.handle.net/11492/8461
dc.identifier.volume219en_US
dc.identifier.wosWOS:001222241900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorİncir, İbrahim
dc.language.isoen
dc.publisherAcademic Press Inc.en_US
dc.relation.journalProtein Expression and Purificationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOptimized Protein Productionen_US
dc.subjectRecombinant Proteinen_US
dc.subjectSolubility Of Proteinen_US
dc.titleEscherichia coli as a versatile cell factory: Advances and challenges in recombinant protein productionen_US
dc.typeArticle

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