Multi-detection of seratonin and dopamine based on an electrochemical aptasensor

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dc.authoridYıldırım-Tirgil, Nimet/0000-0002-5973-8830
dc.contributor.authorCuhadar, Sare Nur
dc.contributor.authorDurmaz, Habibe
dc.contributor.authorYildirim-Tirgil, Nimet
dc.date.accessioned2025-01-12T17:19:39Z
dc.date.available2025-01-12T17:19:39Z
dc.date.issued2024
dc.departmentKMÜ, Mühendislik Fakültesi, Elektrik Elektronik Mühendisliği Bölümü
dc.description.abstractChemical stimuli that enable neurons to communicate with each other are called neurotransmitters. The task of these stimulants is to transmit the messages of neurons to target cells. In this work, a multi-detection mode electrochemical aptasensor system was developed for the neurotransmitters dopamine (3,4-dihydroxyphenylethylamine, DA) and serotonin (5 hydroxytryptamine, SER), which are associated with motivation-based learning, motor control, addiction, activation, arousal, immunity, central nervous systems. These neurotransmitters are related to many diseases, such as depression, primary pulmonary hypertension, kidney disease, Alzheimer's, Huntington's, and Parkinson's. This study used aptamer, a nucleic acid-based biorecognition agent, to capture the target molecules from complex matrix samples. After the capturing procedures, an electrochemical method (differential pulse voltammetry) was performed to observe the specific oxidation profiles of DA and SER. The developed multi-detection mode biosensor system had a linear response from 0.1 to 4 mu M with detection limits of 0.06 mu M for DA and 0.12 mu M for SER. In addition, real serum analyses were performed in the prepared direct electrochemical aptasensor system, and 86-92% of recovery values were observed. As a general conclusion, the main drawback of the multi-detection mode-based methods, sensitivity, can be solved by the aptamer binding process as implemented in this work.
dc.description.sponsorshipScientific and Technological Research Council of TURKIdot;YE (TUBIdot;TAK) [121E346]
dc.description.sponsorshipThe Scientific and Technological Research Council of TURK & Idot;YE (TUB & Idot;TAK), 121E346, Habibe Durmaz
dc.identifier.citationCuhadar, S. N., Durmaz, H., & Yildirim-Tirgil, N. (2024). Multi-detection of seratonin and dopamine based on an electrochemical aptasensor. Chemical Papers, 78(12), 7175–7185. https://doi.org/10.1007/s11696-024-03598-w
dc.identifier.doi10.1007/s11696-024-03598-w
dc.identifier.endpage7185
dc.identifier.issn0366-6352
dc.identifier.issn2585-7290
dc.identifier.issue12
dc.identifier.scopus2-s2.0-85199043290
dc.identifier.scopusqualityQ2
dc.identifier.startpage7175
dc.identifier.urihttps://doi.org/10.1007/s11696-024-03598-w
dc.identifier.urihttps://hdl.handle.net/11492/10135
dc.identifier.volume78
dc.identifier.wosWOS:001272733100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorDurmaz, Habibe
dc.institutionauthoridDurmaz, Habibe/0000-0002-5929-861X
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofChemical Papers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAptasensor
dc.subjectSerotonin detection
dc.subjectDopamine detection
dc.subjectElectrochemical detection
dc.subjectAptamer
dc.subjectBiomarker
dc.titleMulti-detection of seratonin and dopamine based on an electrochemical aptasensor
dc.typeArticle

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