Establishment of efficient micropropagation system in Bishop’s weed (Trachyspermum Ammi L) using seed as explant

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dc.authorid0000-0003-0141-5817en_US
dc.authorid0000-0002-8524-9029en_US
dc.contributor.authorKoca, Ayşe
dc.contributor.authorAasim, Muhammad
dc.date.accessioned2019-12-06T21:16:57Z
dc.date.available2019-12-06T21:16:57Z
dc.date.issued2015
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.descriptionWOS:000353033100022en_US
dc.description.abstractBishop's weed (Trachyspermum ammi L.) is an important medicinal, aromatic and spice plant. The study reports multiple shoot regeneration of bishop's weed using seed explant, cultured on 1.0 x MS medium containing 0.1-1.6 mg l(-1) Kin or TDZ with and without 0.10 mg l(-1) IBA (10 combinations); and 0.5-2.50 mgl(-1) BA with and without 0.10 mg l(-1) IBA (10 combinations). Shoot regeneration ranged 1.73 to 2.14 shoots per explant on 1.0 x MS medium containing variants of Kin with and without IBA. Whereas, multiple shoot induction was recorded on 1.0 x MS medium containing variants of TDZ and variants of BAP with and without IBA that ranged 4.05 to 9.73 and 5.74 to 10.05 shoots per explant respectively. The results confirmed that 1.0xMS medium containing variants of Kin with and without IBA were least suitable for micropropagation purposes. Whereas, 1.0 x MS medium containing variants of BAP with and without IBA induced maximum number of shoots per explant compared to other regeneration media irrespective of the concentration of plant growth regulators. The results further testified successful induction of roots on 1.0 x MS medium containing 1.0 mg l(-1) IBA supplemented with 75 g l(-1) sucrose in growth chamber where the plants set seeds under 16 h day length light with 23 degrees C temprature and 90 % relative humidity after 8 weeks.en_US
dc.identifier.citationKoca, A., Aasim, M.,(2015).Establishment of efficient micropropagation system in Bishop’s weed (Trachyspermum Ammi L) using seed as explant. Journal of Animal and Plant Sciences. 25, 2; 478-484.en_US
dc.identifier.endpage484en_US
dc.identifier.issn1018-7081
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84927547039
dc.identifier.scopusqualityQ3
dc.identifier.startpage478en_US
dc.identifier.urihttps://hdl.handle.net/11492/3019
dc.identifier.volume25en_US
dc.identifier.wosWOS:000353033100022
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorKoca, Ayşe
dc.institutionauthorAasim, Muhammad
dc.language.isoen
dc.publisherPakistan Agricultural Scientists Forumen_US
dc.relation.journalJournal of Animal and Plant Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIn Vitroen_US
dc.subjectZygotic Embryo with Two Cotyledonsen_US
dc.subjectShoot Regenerationen_US
dc.subjectSucroseen_US
dc.subjectAcclimatizationen_US
dc.titleEstablishment of efficient micropropagation system in Bishop’s weed (Trachyspermum Ammi L) using seed as explanten_US
dc.typeArticle

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