Effect of cadmium, chromium, and lead on micropropagation and physio-biochemical parameters of Bacopa monnieri (L.) Wettst. cultured in vitro

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dc.authorid0000-0003-3138-5903en_US
dc.contributor.authorDoğan, Muhammet
dc.date.accessioned2019-12-06T21:15:07Z
dc.date.available2019-12-06T21:15:07Z
dc.date.issued2019
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.descriptionWOS:000468989000011en_US
dc.description.abstractHeavy metals are released into the environment as a result of anthropogenic activities such as melting metallic ores, industrial productions, power stations, and pesticides. Among these, cadmium (Cd), chromium (Cr) and lead (Pb) are the most common heavy metals in the environment and can cause serious toxic effects on both animals and plants. In this study, the effect of Cd, Cr, and Pb on the propagation of Bacopa monnieri (L.) Wettst. by tissue culture was investigated. In addition, various physio-biochemical parameters (photosynthetic pigments contents, protein contents, lipid peroxidation, and hydrogen peroxide) and survival frequency of the plant were evaluated. The shoot tip explants of B. monnieri were cultured in Murashige and Skoog (MS) nutrient media supplemented with 0.50mg/L 6-benzylaminopurine (BAP) and different concentrations of Cd, Cr, and Pb (0.25-1.00mg/kg) for 10weeks. 100% shoot regeneration was determined in MS medium containing 0.25, 0.50, and 0.75mg/kg Cd, 0.25mg/kg Cr and 0.25 and 0.50mg/kg Pb. Mean number of shoots per explant and shoot lengths decreased significantly with increasing metal concentration. In Cd, Cr, and Pb applications, maximum number of shoots per explant was obtained as 30.52, 22.47, and 27.12 shoot/explant and the highest shoot lengths were determined as 4.16, 4.27, and 4.35cm, respectively. As the concentration of the metal exposed to the explant increased, the photosynthetic pigment contents of the plant decreased. The negative impact of metals on photosynthetic pigment contents was found to increase in the order: Cd<Cr<Pb. Similarly, the protein content of plants also decreased due to the negative effect of metals. On the other hand, malondialdehyde (MDA) and hydrogen peroxide (H2O2) values elevated with increasing concentrations of heavy metals. The findings of this study carried out under in vitro conditions may shed light on researchers who want to investigate the effects of heavy metals on plants.en_US
dc.identifier.citationDoğan, M. (2019). Effect of cadmium, chromium, and lead on micropropagation and physio-biochemical parameters of Bacopa monnieri (L.) Wettst. cultured in vitro. Rendiconti Lincei - Scienze Fisiche E Naturali, 30, 2, 351-366.
dc.identifier.doi10.1007/s12210-019-00796-7
dc.identifier.endpage366en_US
dc.identifier.issn2037-4631
dc.identifier.issn1720-0776
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85064276951
dc.identifier.scopusqualityQ2
dc.identifier.startpage351en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s12210-019-00796-7
dc.identifier.urihttps://hdl.handle.net/11492/2437
dc.identifier.volume30en_US
dc.identifier.wosWOS:000468989000011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorDoğan, Muhammet
dc.language.isoen
dc.publisherSpringer-Verlag Italia Srlen_US
dc.relation.journalRendiconti Lincei - Scienze Fisiche E Naturalien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcclimatizationen_US
dc.subjectHeavy Metalen_US
dc.subjectShoot Regenerationen_US
dc.subjectTissue Cultureen_US
dc.subjectToxicityen_US
dc.titleEffect of cadmium, chromium, and lead on micropropagation and physio-biochemical parameters of Bacopa monnieri (L.) Wettst. cultured in vitroen_US
dc.typeArticle

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