Optimization of Sterilization and Micropropagation of Water Lettuce (Pistia stratiotes L.)

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dc.authorid0000-0002-8524-9029en_US
dc.authorid0000-0003-3138-5903en_US
dc.contributor.authorAasim, Muhammad
dc.contributor.authorKarataş, Mehmet
dc.contributor.authorKhawar, Khalid Mahmood
dc.contributor.authorDoğan, Muhammet
dc.date.accessioned2015-02-23T07:27:38Z
dc.date.available2015-02-23T07:27:38Z
dc.date.issued2013
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractWater lettuce (Pistia stratiotes) is an important medicinal aquatic plant that is also used for phytoremediation or as bioindicator. It is very difficult to multiply the plant using micropropagation techniques due to high contaminations. The study aimed to alleviate this problem for efficient multiplication of the plant subsequently. The study made use of 60% commercial bleach (5% NaOCl) or 60% H2O2 for surface sterilization for 10 min followed by adding 500 mg/l Duocid® (a wide spectrum antibiotic) in the culture medium. The results showed that commercial bleach was not proper sterliser as all explants bleached; whereas, 60% H2O2 based surface sterilization was most optimum for the purpose. The Surface sterilized plants were in vitro cultured on agar solidified or liquid MS medium containing 0.05-0.40 mg/l kinetin. Cent percent shoot regeneration frequency was on both agar solidified or liquid medium. However, all explants on agar solidified or liquid medium had tenancy to die turning all of them to albino. Therefore, multiplication under ex vitro conditions on liquid MS medium containing 0.05-0.40 mg/l kinetin or BAP using unsterilized shoot meristems without adding sucrose. Subsequently, the explants induced 2.0-40 plantlets per explant on 2.0-3.0 mg/l kinetin or BAP containing culture medium. The plants regenerated on both culture media were comparable with plants growing under natural conditions and did not show any sign of stress causing albinism or necrosis.en_US
dc.identifier.citationAasim, Muhammad...(ve ark.)"Optimization of Sterilization and Micropropagation of Water Lettuce (Pistia stratiotes L.)"Journal of Applied Biological Sciences, 7,3, 71-74 s., 2013en_US
dc.identifier.endpage77en_US
dc.identifier.issn13071130
dc.identifier.issn21460108
dc.identifier.issue3en_US
dc.identifier.startpage71en_US
dc.identifier.urihttps://hdl.handle.net/11492/1149
dc.identifier.volume7en_US
dc.institutionauthorAasim, Muhammad
dc.institutionauthorKarataş, Mehmet
dc.institutionauthorDoğan, Muhammet
dc.language.isoen
dc.publisherYayıncı Yoken_US
dc.relation.journalJournal of Applied Biological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPlant growth regulatorsen_US
dc.subjectMicropropagationen_US
dc.subjectSterilizationen_US
dc.subjectWater lettuceen_US
dc.titleOptimization of Sterilization and Micropropagation of Water Lettuce (Pistia stratiotes L.)en_US
dc.typeArticle

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