Adventitious shoot regeneration of roundleaf toothcup-rotala rotundifolia [(buch-ham. ex roxb) koehne]

dc.authoridAasim, Muhammad/0000-0002-8524-9029
dc.contributor.authorKarataş, Mehmet
dc.contributor.authorAasim, Muhammad
dc.contributor.authorÇiftçioğlu, M.
dc.date.accessioned2025-01-12T17:20:34Z
dc.date.available2025-01-12T17:20:34Z
dc.date.issued2014
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümü
dc.description.abstractRoundleaf toothcup - Rotala rotundifolia [(Buch-Ham. ex Roxb) Koehne] is an important aquatic, ornamental and medicinal plant of family Lythraceae. Present study was designed to propagate adventitious shoots regeneration from leaf explants cultured on MS medium supplemented with 5 levels of 0.25-2.00 mg/L BA. Maximum shoot regenration frequency (53.33 percent) and mean number of 17.06 shoots per explant was reocrded on MS medium supplemented with 1.0 mg/L BA. Multiple shoots buds were also induced on all culture mediums most of which were either slow to grow or failed to convert in to shoots. Transfer of explants to 0.20 mg/L GA(3) propmoted the shoot regeneration frequecy to 100 percent on MS medium containing 1.00 mg/L BA and increased mean number of shoots (24.0) on MS medium supplemented with 0.25 mg/L BA-0.20 mg/L GA(3). The developing shoots rooted in the shoot elongation medium showed positive effects of BA-0.20 mg/L GA(3) combinations. Thereafter, the developing plantlets were acclimatised in water using pH level of 4.0-10 (7 levels). All plants at pH 4.0 developed necrosis and died; whereas, rest of the plants showed variable grpwth at other pH levels. A comparison among the pH treatments suggested slightly acidic to alkaline medium (pH 6-9) was more appropriate for the acclimatisation of in vitro regenerated plants without having negative effects on plant growth and development.
dc.description.sponsorshipKaramanoglu Mehmetbey University through the Scientific Research Project commission (BAP) [32-M-12]
dc.description.sponsorshipThe Authors acknowledged the Financial assistance by the Karamanoglu Mehmetbey University through the Scientific Research Project commission (BAP) for funding project number 32-M-12.
dc.identifier.citationKaratas M., Aasim M., & Ciftcioglu M. (2014). Adventitious shoot regeneration of roundleaf toothcup-rotala Rotundifolia [(Buch-Ham. ex Roxb) Koehne]. Journal of Animal and Plant Sciences, 24(3), 838–842.
dc.identifier.endpage842
dc.identifier.issn1018-7081
dc.identifier.issue3
dc.identifier.startpage838
dc.identifier.urihttps://hdl.handle.net/11492/10439
dc.identifier.volume24
dc.identifier.wosWOS:000339234900025
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Sceince
dc.language.isoen
dc.publisherPakistan Agricultural Scientists Forum
dc.relation.ispartofJournal of Animal and Plant Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcclimatisation
dc.subjectAdventitous
dc.subjectAquatic
dc.subjectIn vitro
dc.subjectShoots
dc.subjectRegeneration
dc.titleAdventitious shoot regeneration of roundleaf toothcup-rotala rotundifolia [(buch-ham. ex roxb) koehne]
dc.typeArticle

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