Molecular breeding of Selçuklu-97 durum wheat cultivar for some genes affecting pasta quality

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dc.authorid0000-0001-7161-1552en_US
dc.contributor.authorSönmezoğlu, Özlem Ateş
dc.contributor.authorYıldırım, Ahmet
dc.contributor.authorGüleç, T.E.
dc.contributor.authorKandemir, N.
dc.contributor.authorSayaslan, Abdulvahit
dc.contributor.authorKoyuncu, Mehmet
dc.date.accessioned2019-12-06T20:30:05Z
dc.date.available2019-12-06T20:30:05Z
dc.date.issued2010
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe most efficient way of producing a nd supplying proper raw material desired by the pasta industry is the development of high quality durum wheat varieties. Therefore, quality of Turkish durum wheat varieties should be improved using modern breeding methods without adversely affecting their yields. In this study, important genes (y-gliadin 45 and LMW-2 glutenin) affecting the quality of pasta products were transferred to a Turkish durum wheat variety, Selçuklu-97, in a backcross breeding method in combination with marker assisted selection (MAS). A Canadian durum wheat cultivar with high quality, Kyle, was used as the donor parent. Each of F1 and backcross (BC) plants was backcrossed four times to the recurrent parent and in all of the generations, backcrossed plants carrying the targeted QTLs were selected by the MAS. The MAS method was employed in combination with embryo culture and rapid plant growth in a controlled greenhouse conditions. In identifying and transferring processes of the gene regions, molecular DNA markers (SSR, STS and GAG) were employed with A-PAGE and SDS-PAGE methods. A-PAGE was used for selection of y-gliadin 45, SDS-PAGE for selection of LMW-2 glutenins, and four SSR primers (Xgwm550, Xgwm608, Stm553actc, Stm542acag), one STS primer and one PCR primer (GAG5-6) linked to Gli-B1 and Glu-B3 loci for selection of y-gliadin 45 and LMW-2 glutenins either all together or alternatively. As a result, the study was completed in three years instead of six years required in a classical backcross breeding study, meaning about 50 % time saving, and obtained a high quality candidate variety.en_US
dc.identifier.citationSönmezoğlu, Ö. A., Güleç, T. E., Kandemir, N., Yıldırım, A., Sayaslan, A., Koyuncu, M. (2010). Molecular breeding of Selçuklu-97 durum wheat cultivar for some genes affecting pasta quality. Journal of Applied Biological Sciences, 4, 2, 46-50.
dc.identifier.endpage50en_US
dc.identifier.issn1307-1130
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-79957550638
dc.identifier.scopusqualityN/A
dc.identifier.startpage46en_US
dc.identifier.urihttps://hdl.handle.net/11492/1959
dc.identifier.volume4en_US
dc.indekslendigikaynakScopus
dc.institutionauthorYıldırım, Ahmet
dc.institutionauthorSayaslan, Abdulvahit
dc.language.isoen
dc.relation.journalJournal of Applied Biological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectA-PAGEen_US
dc.subjectBackcross Breedingen_US
dc.subjectDurum Wheaten_US
dc.subjectLMW-2 Gluteninen_US
dc.subjectSDS-PAGEen_US
dc.subjectSSRen_US
dc.subjectTriticum Durumen_US
dc.subjectY-Gliadin 45en_US
dc.titleMolecular breeding of Selçuklu-97 durum wheat cultivar for some genes affecting pasta qualityen_US
dc.typeArticle

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