Micropropagation Of Lentil (Lens Culinaris Medik.) Using Pulse Treatment Of İmmature Plumular Apices

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dc.authorid0000-0002-8524-9029en_US
dc.contributor.authorAasim, Muhammad
dc.date.accessioned2015-02-20T12:25:39Z
dc.date.available2015-02-20T12:25:39Z
dc.date.issued2012
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractLentil is highly recalcitrant and is difficult to regenerate through tissue culture. The study is aimed to overcome this problem by developing an efficient regeneration system using immature plumular apice explants from immature zygotic embryos of Turkish lentil cv. Ciftci. The results showed that 10 mg/l BA pulse treatment of explants for 10 days followed by culture on MS medium containing various concentrations of BA-IBA supplemented with activated charcoal and PVP affected shoot regeneration frequency, mean number of shoots per explant and shoot length. Irrespective of the pulse treatment, combination of BA with IBA in MS medium promoted longer shoots compared to any concentration of BA alone. Maximum number of shoots (4.25) per explant was recorded on MS medium containing 0.25 mg/l BA + 0.1 mg/l IBA after pulse treatment. The longest shoots (6.17 cm) were recorded in pulse treated explants when cultured on MS medium containing 0.25 mg/l BA + 0.1 mg/l IBA. The regenerated shoots were rooted on MS medium containing 0.25 to 1 mg/l IBA or 1 mg/l IAA. The rooted plants were acclimatized at 24±2oC in the growth room where, they flowered and set seeds.en_US
dc.identifier.citationAasim, Muhammad"Micropropagation Of Lentil (Lens Culinaris Medik.) Using Pulse Treatment Of İmmature Plumular Apices"Pak. J. Agri. Sci.,49,2, 149-154 s.,2012.en_US
dc.identifier.endpage154en_US
dc.identifier.issn05529034
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84864046592
dc.identifier.scopusqualityQ3
dc.identifier.startpage149en_US
dc.identifier.urihttps://hdl.handle.net/11492/1142
dc.identifier.volume49en_US
dc.indekslendigikaynakScopus
dc.institutionauthorAasim, Muhammad
dc.language.isoen
dc.publisherYayıncı Yoken_US
dc.relation.journalPak. J. Agri. Sci.en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPlumular apicesen_US
dc.subjectExplanten_US
dc.subjectLegumeen_US
dc.subjectLentilen_US
dc.subjectShoot regenerationen_US
dc.subjectGrowth regulatorsen_US
dc.titleMicropropagation Of Lentil (Lens Culinaris Medik.) Using Pulse Treatment Of İmmature Plumular Apicesen_US
dc.typeArticle

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