Adventitous bulblet regeneration of endemic Ovacik garlic (Allium tuncelianum Kollman, Özhatay, Mathew, Şiraneci) using wintered half clove explant
Yükleniyor...
Dosyalar
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ARS Docendi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Ovacik garlic (Allium tuncelianum Kollman, Ozhatay, Mathew, Siraneci) is an important endemic and threatened plant species of Eastern Turkish province of Tunceli and its surrounding areas with poor seed set and germination problems. There is an urgent need to develop strategies for protection and conservation of the plant by the development of in vitro conservation protocols. Hence, the present study was focused to develop an efficient regeneration protocol of wintered and unwintered half cloves of the Ovacik garlic that were cultured on 0.25, 0.50 and 1.0 mg L-1 Benzylaminopurine (BA) with 0.25, 0.50 and 1.0 mg L-1 of Potassium salt of Naphthaleneacetic acid (KNAA) for regeneration. Unwintered upper and lower half clove explants failed to regenerate new bulblets. Similarly, no regeneration was recorded on wintered upper half of the Ovacik garlic. Proximal half clove of the wintered Ovacik garlic was evaluated as best explant for regeneration on MS medium containing 0.50 mg L-1 BA with 0.50 mg L-1 KNAA. Regenerated bulblets were rooted on MS medium supplemented with 60 g L-1 sucrose. The rooted bulbs were acclimatized and transferred to pots and fields. It was concluded that the protocol could be safely used to conserve this plant.
Açıklama
WOS:000365405800013
Anahtar Kelimeler
Adventitous, Clove, Endemic, Garlic, Medicinal
Kaynak
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
20
Sayı
5
Künye
Aasim, M. (2015). Adventitous bulblet regeneration of endemic Ovacik garlic (Allium tuncelianum Kollman, Özhatay, Mathew, Şiraneci) using wintered half clove explant. Roumanian Biotechnological Letters, 20, 5, 10845-10851.
