In vitro shoot regeneration from preconditioned explants of chickpea (Cicer arietinum L.) cv. Gökçe
Yükleniyor...
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Journals
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present study reports the successful shoot regeneration of preconditioned mature embryo and embryonic axis explants of chickpea cv. Gokce. Explants were preconditioned with 10 mgl benzylaminopurine (BA) for 7 days followed by culture on Murashige and Skoog (MS) medium containing 0.25, 0.50, 1.00 and 2.00 mg/l BA with or without 0.25 mg/l naphthalene acetic acid (NAA) supplemented with 4 mg/l activated charcoal and 1 mg/l polyvinylpyrrolidon (PVP). Shoot regeneration was recorded on all explants. Maximum number of (14.75) shoots per explants on mature embryo were recorded on MS medium containing 2.0 mg/l BA with 0.25 mg/l NAA. Whereas, 16.83 shoots per explants were recorded on MS medium containing 2.0 mg/l BA. Presence of NAA in the culture medium decreased the mean shoot length of both explants compared to medium devoid of 0.25 mg/l NAA. Regenerated shoots were rooted on MS medium containing 1.0 mg/l indole-butyric acid (IBA) after 4 weeks of culture. Rooted plantlets were transferred to pots for acclimatization under green house conditions.
Açıklama
WOS:000290093900007
Anahtar Kelimeler
In Vitro, Chickpea, Pulse Treatment, Shoot Regeneration
Kaynak
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
10
Sayı
11
Künye
Aasim, M., Day, S., Rezaei, F., Hajyzadeh, M., Ozcan, S., Mahmud, S. T. (2011). In vitro shoot regeneration from preconditioned explants of chickpea (Cicer arietinum L.) cv. Gökçe. African Journal of Biotechnology, 10, 11, 2020-2023.
