Selection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assay

Basit Öğe Kaydı
dc.authorid0000-0002-8049-7867en_US
dc.contributor.authorBayraç, Abdullah Tahir
dc.contributor.authorDönmez, Sultan İlayda
dc.date.accessioned2019-12-06T21:15:28Z
dc.date.available2019-12-06T21:15:28Z
dc.date.issued2018
dc.departmentKMÜ, Mühendislik Fakültesi, Biyomühendislik Bölümüen_US
dc.descriptionWOS:000441368000014en_US
dc.descriptionPubMed:29964028en_US
dc.description.abstractPneumococci are one of the leading causes of infections throughout the world causing problems mainly in children, elderly, and immune-deficient patients. In recent years antibiotic resistant Streptococcus pneumoniae strains become widespread. Therefore simple, rapid, and specific detection methods are needed for public health. In this study, DNA aptamer probes against S. pneumoniae were selected using bacterial Systematic Evolution of Ligands by Exponential Enrichment (SELEX) and these probes were integrated in to a graphene oxide (GO) based fluorescent assay. Among the tested aptamers three candidates Lyd-1, Lyd-2 and Lyd-3 showed K-d values of 844.7 +/- 123.6, 1984.8 +/- 347.5, and 661.8 +/- 111.3 nM, respectively. These candidates showed binding affinity to S. pneumoniae and no specific binding to the bacteria used in negative selection. The binding of aptamers were showed by fluorescence spectroscopy and flow cytometry. GO based label-free fluorescent assay developed using Lyd-3 aptamer had a unique detection limit of 15 cfu mL(-1) . Thus we believe that the selected aptamers and fabricated GO based assay has potential to be used in the detection of S. pneumoniae. Selected aptamers selectively bind to S. pneumonia with anti-pneumococcal potential and holds great potential to be used as molecular probes for identifying and targeting.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114S856]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey [Project No. 114S856].en_US
dc.identifier.citationBayraç, A. T., Dönmez, S. I. (2018). Selection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assay. Analytical Biochemistry, 556, 91-98.
dc.identifier.doi10.1016/j.ab.2018.06.024
dc.identifier.endpage98en_US
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid29964028
dc.identifier.scopus2-s2.0-85049336454
dc.identifier.scopusqualityQ2
dc.identifier.startpage91en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ab.2018.06.024
dc.identifier.urihttps://hdl.handle.net/11492/2601
dc.identifier.volume556en_US
dc.identifier.wosWOS:000441368000014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBayraç, Abdullah Tahir
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.journalAnalytical Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAptameren_US
dc.subjectPneumoniaeen_US
dc.subjectSelexen_US
dc.subjectPathogen Biosensoren_US
dc.subjectBiofilmen_US
dc.titleSelection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assayen_US
dc.typeArticle

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