A new organic dye-based staining for the detection of plant dna in agarose gels
Yükleniyor...
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Ethidium bromide (EtBr) is used to stain DNA in agarose gel electrophoresis, but this dye is mutagenic and carcinogenic. We investigated N-719, which is a visible, reliable and organic Ruthenium-based dye, and five fluorescent alternatives for staining plant DNA. For prestaining and poststaining, N-719, GelRed, and SYBR Safe stained both DNA and PCR product bands as clearly as EtBr. SYBR Green I, methylene blue, and crystal violet were effective for poststaining only. The organic dye N-719 stained DNA bands as sensitively and as clearly as EtBr. Consequently, organic dyes can be used as alternatives to EtBr in plant biotechnology studies.
Açıklama
WOS:000357650600005
PubMed: 26158569
PubMed: 26158569
Anahtar Kelimeler
Organic Dye, Gel Electrophoresis, DNA Staining
Kaynak
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
34
Sayı
7
Künye
Sönmezoğlu, Ö. A., Özkay, K. (2015) A new organic dye-based staining for the detection of plant dna in agarose gels. Nucleosides, Nucleotides and Nucleic
Acids, 34:7, 515-522.
