In vitro high frequency regeneration through apical shoot proliferation of Hemianthus callitrichoides 'Cuba' - a multipurpose ornamental aquatic plant
Yükleniyor...
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technical Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Hemianthus callitrichoides 'Cuba' is an ornamental aquatic plant used in water gardens and phytoremediation. A protocol for its rapid and reproducible shoot organogenesis from apical shoot explants was developed. Optimum culture conditions for shoot proliferation were tested in MS media containing different concentrations of 6-(gamma,gamma-dimethylallylamino)purine (2iP), 6-benzylaminopurine (BAP), 1-phenyl-3-1,2,3-thiadiazol-5-yl urea (TDZ), and gibberellic acid (GA(3)). Plant growth regulators significantly (P <= 0.001) affected the diameter, area, and fresh weight of shoot clumps. On the basis of plant growth, the maximum shoot diameter (3.06 cm), area (6.12 cm), and clump weight (2.85 g) of apical shoots were achieved on MS medium containing 0.50 mg L-1 BAP. Higher concentrations of sucrose (9%) showed visible phenotypic growth inhibition and abnormalities were observed in vitro. The regenerated shoots rooted efficiently on MS medium without growth regulators. The growth pattern of multiple shoots indicated their origin from an enlarged shoot base via the proliferation of apical shoots. All in vitro regenerated plantlets acclimatized in aquariums successfully. The simplicity of the protocol and the direct production of multiple shoots make it a potential system that is highly amenable to true-to-type plant regeneration with the ability to maintain genetic stability.
Açıklama
WOS:000356358200017
Anahtar Kelimeler
Aquatic Plant, Apical Shoot, Hemianthus Callitrichoides, In Vitro, Regeneration, Proliferation, Plant Growth Regulators
Kaynak
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
39
Sayı
3
Künye
Barpete, S., Özcan, S. F., Aasim, M., Özcan, S. (2015). In vitro high frequency regeneration through apical shoot proliferation of Hemianthus callitrichoides `Cuba. Turkish Journal of Biology, 3, 39, 493-500.
