Single laboratory validation of four methods for quantification of HMF in honey

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dc.contributor.authorYıldız, Elif
dc.contributor.authorBayraç, Abdullah Tahir
dc.date.accessioned2025-01-12T17:14:33Z
dc.date.available2025-01-12T17:14:33Z
dc.date.issued2024
dc.departmentKMÜ, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractHydroxymethylfurfural or 5-(hydroxymethyl)-2-furaldehyde (HMF) has been absent or found in honey naturally at very low amount. It is formed in honey mainly by heating process and improper storage conditions. HMF has been reported to have cytotoxic, carcinogenic, and mutagenic effects and thus regulatory agencies in many countries impose restrictions on its maximum levels in honey. Validated methods have been required for effective and specific detection and quantification of HMF in food samples. In this study, a single laboratory validation study was conducted on four quantification methods: direct spectral analysis, High Performance Liquid Chromatography (HPLC) analysis, Seliwanoff and Winkler methods. All methods showed linearity with the lowest R2 value of 0.992. Two method performance parameters, accuracy, and precision were satisfied by each four methods with recovery values at 98.2%, 100.2%, 102.5% and 103.3% and RSDr (relative standard deviation) % values at 6.97%, 6.19%, 2.87% and 0.90% for spectral analysis, Seliwanoff, HPLC and Winkler methods, respectively. Based on the measurement uncertainties of four quantification methods, honey samples spiked with HMF at the final concentration of 0.004mg/0.1g were reported as 0.004 mg/0.1g ± 0.00025 mg/0.1g by spectral analysis, 0.0036 mg/0.1g ± 0.000691 mg/0.1g by Seliwanoff method, 0.004 mg/0.1g ± 0.00045 mg/0.1g by HPLC and 0.0039 mg/0.1g ± 0.00022 mg/0.1g by Winkler methods (k=2, confidence level of 95%). The validated methods can quantify HMF in honey with a target concentration of 0.004 mg/0.1g, specifically and accurately.
dc.identifier.citationGür, E. Y., Bayraç, A. T., Bayraç, A. T. (2024). Single laboratory validation of four methods for quantification of HMF in honey. Journal of advanced research in natural and applied sciences (Online), 10(1),45-59. https://doi.org/10.28979/jarnas.1340071
dc.identifier.doi10.28979/jarnas.1340071
dc.identifier.endpage59
dc.identifier.issn2757-5195
dc.identifier.issue1
dc.identifier.startpage45
dc.identifier.trdizinid1228860
dc.identifier.urihttps://doi.org/10.28979/jarnas.1340071
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1228860
dc.identifier.urihttps://hdl.handle.net/11492/9940
dc.identifier.volume10
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorBayraç, Abdullah Tahir
dc.institutionauthoridBayraç, Abdullah Tahir/0000-0002-8049-7867
dc.language.isoen
dc.relation.ispartofJournal of advanced research in natural and applied sciences (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHMF
dc.subjectuncertainty
dc.subjecthoney
dc.subjectmethod validation
dc.subjectSeliwanoff
dc.titleSingle laboratory validation of four methods for quantification of HMF in honey
dc.typeArticle

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