Nöromüsküler yorgunluğun sinaptik iletide rol alan asetilkolin üzerine etkisi
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Dosyalar
Tarih
2024
Yazarlar
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Yayıncı
Karamanoğlu Mehmetbey Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı nöromüsküler yorgunluk sonrası sinaptik iletide rol alan ACh seviyelerindeki değişimin incelenmesidir. Araştırmaya Karamanoğlu Mehmetbey Üniversitesi Spor Bilimleri Fakültesinde haftada en az 3 kez rekreatif olarak spor yapan 15 erkek öğrenci katılmıştır (yaş=22,26±1,16 yıl, boy=175,8±6,46 cm, vücut ağırlığı=78,76±12,99 kg, BKİ=25,51±4,34 kg/m2). Çalışmada kontrol grupsuz yarı deneysel araştırma deseni uygulanmıştır. Katılımcılara nöromüsküler yorgunluk oluşturmak için 30-15 IFT uygulaması yaptırılmıştır. 30-15 IFT öncesinde ve sonrasında katılımcılardan kan örnekleri alınarak Asetilkolin (ACh), Asetilkolinesteraz (AChE) ve kolin değişkenleri incelenmiştir. Elde edilen verilerin normal dağılım gösterip göstermediği çarpıklık basıklık değerleri incelenmiş ve verilerin normal dağıldığı sonucuna ulaşılmıştır. Test-tekrar test güvenilirliği, sınıf içi korelasyon katsayısı (ICC), ön ve son test arasındaki değişimi belirlemek için bağımlı örneklem t-testi ve değişkenler arası ilişki için Pearson's korelasyon analizi kullanılmıştır. Verilerin analizinde SPSS 27 paket programında yapılmış ve anlamlılık düzeyi p<0,05 olarak belirlenmiştir. Yapılan analizler sonunda ACh, AChE ve kolin ön test ve son test değerleri arasında anlamlı farklılık olmadığı bulunmuştur (p>0,05). Katılımcıların en güvenilir testi ACh olup ICC değeri 0,914 ve yüksek seviyede olduğu bulunmuştur. AChE ve kolin ICC değerleri orta düzeyde sırasıyla 0,696 ve 0,726 olarak saptanmıştır. Değişkenlerin Pearson korelasyon test sonuçlarına göre, ACh ve kolin arasında (r=,664, p<0,01) pozitif ilişki olduğu sonucuna ulaşılmıştır. Sonuç olarak ACh, AChE ve kolin seviyelerinin nöromüsküler yorgunluğun tanımlanmasında kullanabileceği ve farklı çalışma gruplarıyla desteklenmesi sonucuna ulaşılabilir.
The aim of this study was to examine the changes in ACh levels that contribute to synaptic transmission after neuromuscular fatigue. The study included a sample of 15 male students (mean age=22.26±1.16 years, mean height =175.8±6.46 cm, mean body weight=78.76±12.99 kg, mean BMI =25.51±4.34 kg/m2) who participated in recreational sports at least three times per week at Karamanoğlu Mehmetbey University Faculty of Sports Sciences. The study used a quasi-experimental research methodology that did not include a control group. The subjects performed in a 30-15 intermittent fitness test (IFT) in order to cause neuromuscular fatigue. Participants had blood drawn both before and after 30–15 IFT, and the variables for choline, Acetylcholine (ACh), and Acetylcholinesterase (AChE) were evaluated. The skewness and kurtosis values were analyzed to determine if the data exhibited a normal distribution. It was determined that the data did indeed follow a normal distribution. The study used test-retest reliability, intraclass correlation coefficient (ICC), dependent sample t-test to assess the difference between pre and post-test, and Pearson's correlation analysis to investigate the relationship among variables. The data were analyzed utilizing the SPSS 27 software package, with a significance threshold set at p<0.05. Following concluding the analysis, it was determined that there was no notable disparity between the pre-test and post-test levels of ACh, AChE, and choline (p>0.05). The participants' reliability was assessed using ACh, obtaining an ICC score of 0.914, indicating a good level of reliability. The AChE and choline ICC values were determined to be 0.696 and 0.726, respectively, at a moderate level. The Pearson correlation test results show a positive relationship between ACh and choline (r=0.664, p<0.01). In conclusion, it can be concluded that ACh, AChE and choline levels can be used to define neuromuscular fatigue and should be supported by different study groups.
The aim of this study was to examine the changes in ACh levels that contribute to synaptic transmission after neuromuscular fatigue. The study included a sample of 15 male students (mean age=22.26±1.16 years, mean height =175.8±6.46 cm, mean body weight=78.76±12.99 kg, mean BMI =25.51±4.34 kg/m2) who participated in recreational sports at least three times per week at Karamanoğlu Mehmetbey University Faculty of Sports Sciences. The study used a quasi-experimental research methodology that did not include a control group. The subjects performed in a 30-15 intermittent fitness test (IFT) in order to cause neuromuscular fatigue. Participants had blood drawn both before and after 30–15 IFT, and the variables for choline, Acetylcholine (ACh), and Acetylcholinesterase (AChE) were evaluated. The skewness and kurtosis values were analyzed to determine if the data exhibited a normal distribution. It was determined that the data did indeed follow a normal distribution. The study used test-retest reliability, intraclass correlation coefficient (ICC), dependent sample t-test to assess the difference between pre and post-test, and Pearson's correlation analysis to investigate the relationship among variables. The data were analyzed utilizing the SPSS 27 software package, with a significance threshold set at p<0.05. Following concluding the analysis, it was determined that there was no notable disparity between the pre-test and post-test levels of ACh, AChE, and choline (p>0.05). The participants' reliability was assessed using ACh, obtaining an ICC score of 0.914, indicating a good level of reliability. The AChE and choline ICC values were determined to be 0.696 and 0.726, respectively, at a moderate level. The Pearson correlation test results show a positive relationship between ACh and choline (r=0.664, p<0.01). In conclusion, it can be concluded that ACh, AChE and choline levels can be used to define neuromuscular fatigue and should be supported by different study groups.
Açıklama
Sağlık Bilimleri Enstitüsü, Spor Bilimleri Ana Bilim Dalı
Anahtar Kelimeler
Fizyoloji, Physiology, Spor
