Calixarene-immobilized monolithic cryogels for preparative protein chromatography

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dc.authorid0000-0002-0416-2870en_US
dc.contributor.authorGüven, İdris
dc.contributor.authorGezici, Orhan
dc.contributor.authorBayrakcı, Mevlüt
dc.contributor.authorMorbidelli, Massimo
dc.date.accessioned2019-12-06T21:15:30Z
dc.date.available2019-12-06T21:15:30Z
dc.date.issued2018
dc.departmentKMÜ, Mühendislik Fakültesi, Biyomühendislik Bölümüen_US
dc.descriptionWOS:000436220900007en_US
dc.descriptionPubMed:29778445en_US
dc.description.abstractNew cation exchanger monolithic stationary phases were prepared by immobilization of three different calixarene derivatives (i.e. tetracarboxylate calix[4]arene, CLX-COO, tetrasulfonate calix[4]arene, CLX-SO3, and tetraphosphonate calix[4]arene, CLX-PO4) onto a monolithic cryogel support (i.e. poly(2-hydroksyethylmethacrilate-co-glycidyl methacrylate, P) and investigated with respect to preparative protein chromatography. The obtained monoliths were characterized through various techniques such as FTIR spectroscopy, isoelectric point measurements, titrimetric analyses, and mercury intrusion porosimetry. Protein retention was investigated using some model proteins (i.e. lysozyme, cytochrome c, and alpha-chymotrypsinogen A, human serum albumin, and myoglobin), and the role of modifier (i.e. NaCI) concentration and pH was thoroughly analyzed under isocratic and gradient elution conditions. Overloading experiments were also conducted to study dynamic adsorption capacity and the obtained values were found to be ranging between 3 and 8 mg/mL depending on the type of calixarene molecule. Hence, higher or comparable protein adsorption capacities were seen to be applicable on calixarene-immobilized cryogels when compared to any other functionalized cryogels in the literature. Combined with the favorable properties of these monoliths, with respect to mass transport of large molecules, these results qualify calixarene functionalized monolithic cryogels as promising stationary phases for protein preparative purification. (C) 2018 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [115Z236]en_US
dc.description.sponsorshipAuthors wish to thank TUBITAK (The Scientific and Technological Research Council of Turkey) for the financial support provided (Project number: 115Z236).en_US
dc.identifier.citationGüven, I., Gezici, O., Bayrakcı, M., Morbidelli, M. (2018). Calixarene-immobilized monolithic cryogels for preparative protein chromatography. Journal of Chromatography A, 1558, 59-68.
dc.identifier.doi10.1016/j.chroma.2018.05.026
dc.identifier.endpage68en_US
dc.identifier.issn0021-9673
dc.identifier.issn1873-3778
dc.identifier.pmid29778445
dc.identifier.scopus2-s2.0-85046865653
dc.identifier.scopusqualityQ1
dc.identifier.startpage59en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.chroma.2018.05.026
dc.identifier.urihttps://hdl.handle.net/11492/2617
dc.identifier.volume1558en_US
dc.identifier.wosWOS:000436220900007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBayrakcı, Mevlüt
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.journalJournal of Chromatography Aen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCalixareneen_US
dc.subjectCation-Exchangeen_US
dc.subjectCryogelen_US
dc.subjectMonolithic Columnen_US
dc.subjectSeparationen_US
dc.subjectStationary Phaseen_US
dc.titleCalixarene-immobilized monolithic cryogels for preparative protein chromatographyen_US
dc.typeArticle

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