L-Dopa Synthesis Catalyzed by Tyrosinase Immobilized In Poly(ethyleneoxide) Conducting Polymers

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dc.authorid0000-0003-1123-8797en_US
dc.authorid0000-0001-5865-7687en_US
dc.contributor.authorYıldız, Hüseyin Bekir
dc.contributor.authorÇalışkan, Salim
dc.contributor.authorKamacı, Musa
dc.contributor.authorÇalışkan, Abdullah
dc.contributor.authorYılmaz, Haşim
dc.date.accessioned2014-10-21T11:51:20Z
dc.date.available2014-10-21T11:51:20Z
dc.date.issued2013
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Kimya Bölümüen_US
dc.description.abstract1-3,4-Dihydroxy phenylalanine called as l-Dopa is a precursor of dopamine and an important neural message transmitter and it has been a preferred drug for the treatment of Parkinson’s disease. In this study, with regards to the synthesis of l-Dopa two types of biosensors were designed by immobilizing tyrosinase on conducting polymers: thiophene capped poly(ethyleneoxide)/polypyrrole (PEO-co-PPy) and 3-methylthienyl methacrylate-co-p-vinylbenzyloxy poly(ethyleneoxide)/polypyrrole (CP-co-PPy). PEO-co-PPy and CP-co-PPy were synthesized electrochemically and tyrosinase immobilized by entrapment during electropolymerization. l-Tyrosine was used as the substrate for l-Dopa synthesis. The kinetic parameters of the designed biosensors, maximum reaction rate of the enzyme (Vmax) and Michaelis Menten constant (Km) were determined. Vmax were found as 0.007 mol/(min electrode) for PEO-co-PPy matrix and 0.012 mol/(min electrode) for CP-co-PPy matrix. Km values were determined as 3.4 and 9.2 mM for PEO-co-PPy and CP-co-PPy matrices, respectively. Optimum temperature and pH, operational and shelf life stabilities of immobilized enzyme were also examined.en_US
dc.identifier.citationYıldız, H. Bekir, Çalışkan, Salim, Kamacı, Musa, Çalışkan, Abdullah,Yılmaz, Haşim. "International Journal of Biological Macromolecules.”, International Journal of Biological Macromolecules 56, 34-40,2013en_US
dc.identifier.scopusscopusidyok
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2013.01.031
dc.identifier.urihttps://hdl.handle.net/11492/909
dc.identifier.woswosidyok
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorYıldız, Hüseyin Bekir
dc.institutionauthorKamacı, Musa
dc.language.isoen
dc.publisherInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectConducting polymersen_US
dc.subjectElectropolymerizationen_US
dc.subjectBiocatalysisen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectl-Dopa synthesisen_US
dc.titleL-Dopa Synthesis Catalyzed by Tyrosinase Immobilized In Poly(ethyleneoxide) Conducting Polymersen_US
dc.typeArticle

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