In vitro cytotoxicity of compounds isolated from Desbordesia glaucescens against human carcinoma cell lines

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dc.authorid0000-0003-2028-7339en_US
dc.contributor.authorKuete, V.
dc.contributor.authorMafodong, F. L. Dongmo
dc.contributor.authorÇelik, İ.
dc.contributor.authorFobofou, S. A. T.
dc.contributor.authorNdontsa, B. L.
dc.contributor.authorKaraosmanoğlu, Oğuzhan
dc.contributor.authorWeissjohann, L. A.
dc.date.accessioned2019-12-06T21:15:56Z
dc.date.available2019-12-06T21:15:56Z
dc.date.issued2017
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.descriptionWOS:000405251800006en_US
dc.description.abstractMalignancies constitute a global health concern and chemotherapy remains the main mode of treatment. The present study was designed to evaluate the cytotoxicity of 8 compounds from Desbordesia glaucescens namely lanosta-7,24-dien-3-one (1), friedelanone (2), friedelanol (3), 3,3'-di-O-methylellagic acid (4), 3,3',4'-tri-0-methylellagic acid (5), ellagic acid (6), 3',4'-di-0-methylellagic acid 4-0-beta-o-glucopyranoside (7) and 3,3'-di-0-methylellagic acid 4'-0-beta-c-xylopyranoside (8) against 4 human carcinoma cell lines and normal CRL2120 fibroblasts. The neutral red uptake (NRU) assay was used for cytotoxicity testing. Caspase-Glo assay, cell cycle analysis, measurements of mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were used to evaluate apoptosis induction. Compounds 4 and 6 as well as doxorubicin had IC50 values below 45 pM in the four tested cancer cell lines meanwhile other compounds displayed selective activity. The IC50 values ranged from 11.23 mu M (towards breast adenocarcinoma MCF-7 cells) to 44.65 mu M (colon carcinoma Caco-2 cells) for 4, from 14.07 mu M (towards MCF-7 cells) to 77.73 mu M (Caco-2 cells) for 6 and from 0.07 mu M (towards SPC212 cells) to 1.01 mu M (A549 cells) for doxorubicin. Compound 4 induced apoptosis in MCF-7 cells mediated by MMP loss. The constituents of Desbordesia glaucescens and especially ellagic acid (6) and its derivative 4 are potential cytotoxic compounds that deserve more investigations towards developing novel antiproliferative drugs against human carcinoma. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Counsel of Turkey (TUBITAK); Scientific Research Projects Commission of Anadolu University, Eskisehir, Turkey [1507F563, 1306F110]; International Science Programme, Uppsala University, Sweden (ISP) [KEN-02]en_US
dc.description.sponsorshipV.K. and H.S. are thankful to Scientific and Technological Research Counsel of Turkey (TUBITAK) for 6 months travel grant (to V.K.) and to Scientific Research Projects Commission of Anadolu University, Eskisehir, Turkey for the funding grant 1507F563 (to V.K. and H.S.). A grant for part of this work was also provided by International Science Programme, Uppsala University, Sweden (ISP)-KEN-02 project. IC would like to thank the Scientific Research Projects Commission of Anadolu University, Eskisehir, Turkey for the funding grant (1306F110). Authors are also thankful to Sennur Gorgulti for FACS measurements.en_US
dc.identifier.citationKuete, V., Dongmo, M. F. L., Celik, I., Fobofou, S. A. T., Ndontsa, B. L., Karaosmanoğlu, O. (2017). In vitro cytotoxicity of compounds isolated from Desbordesia glaucescens against human carcinoma cell lines. South African Journal of Botany, 111, 37-43.
dc.identifier.doi10.1016/j.sajb.2017.03.031
dc.identifier.endpage43en_US
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.scopus2-s2.0-85015628518
dc.identifier.scopusqualityQ1
dc.identifier.startpage37en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.sajb.2017.03.031
dc.identifier.urihttps://hdl.handle.net/11492/2765
dc.identifier.volume111en_US
dc.identifier.wosWOS:000405251800006
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorKaraosmanoğlu, Oğuzhan
dc.language.isoen
dc.publisherElsevier Science BVen_US
dc.relation.journalSouth African Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectCarcinomaen_US
dc.subjectCytotoxicityen_US
dc.subjectEllagic Aciden_US
dc.subjectTerpenoidsen_US
dc.subjectMode Of Actionen_US
dc.titleIn vitro cytotoxicity of compounds isolated from Desbordesia glaucescens against human carcinoma cell linesen_US
dc.typeArticle

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