Enhancing in vitro micropropagation of Alternanthera reineckii Briq. using various light-emitting diodes, culture media and plant growth regulators

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dc.contributor.authorEkin, Ümmügülsüm
dc.contributor.authorDoğan, Muhammet
dc.date.accessioned2025-01-12T17:19:43Z
dc.date.available2025-01-12T17:19:43Z
dc.date.issued2024
dc.departmentKMÜ, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü
dc.description.abstractIn this study, the effects of various light treatments, growth regulators and culture media (agar -solidified and liquid) on in vitro propagation of Alternanthera reineckii Briq. Were evaluated. In in vitro experiments were used red, blue, and white LEDs alone and various combinations of red and blue LEDs. Different levels of 6-benzylaminopurine (BAP) and Kinetin (KIN), ranging from 0.25 mg/L to 1 mg/L, were introduced into the solid and liquid Murashige and Skoog (MS) basal medium. In solid culture, the highest number of shoots per explant (16.67 shoots/explant) was observed when utilizing a proportion of 1:2 red/blue LEDs in MS medium supplemented with 1 mg/L BAP. On the other hand, in the liquid culture, 15.13 shoots/explant were obtained with 1 mg/L BAP under a 2:1 red/blue LEDs configuration. During the experiments involving KIN, 10.42 shoots/explant were observed in the solid culture conditions with 1 mg/L of KIN under a lighting configuration of 1:2 red/blue LEDs. On the other hand, a total of 10.62 shoots/explant was attained in the liquid MS medium with 0.50 mg/L of KIN under a lighting configuration of 2:1 red/blue LEDs. As a result, LED lights were found to be better than fluorescent lights for multiple propagations of A. reineckii. Moreover, higher shoot numbers and longer shoots were achieved with red and blue LED light combinations.
dc.description.sponsorshipScientific Research Projects Commission of the Karamanogylu Mehmetbey University [12-YL-20]
dc.description.sponsorshipFunding This work was supported by Scientific Research Projects Commission of the Karamanogylu Mehmetbey University (Project Number: 12-YL-20) .
dc.identifier.citationEkin, U., & Dogan, M. (2024). Enhancing in vitro micropropagation of Alternanthera reineckii Briq. using various light-emitting diodes, culture media and plant growth regulators. Kuwait Journal of Science, 51(3). https://doi.org/10.1016/j.kjs.2024.100250
dc.identifier.doi10.1016/j.kjs.2024.100250
dc.identifier.issn2307-4108
dc.identifier.issn2307-4116
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85193684941
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.kjs.2024.100250
dc.identifier.volume51
dc.identifier.wosWOS:001244107700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthoridDoğan, Muhammet/0000-0003-3138-5903
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofKuwait Journal of Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIn vitro propagation
dc.subjectNodal explant
dc.subjectRooting
dc.subjectShoot regeneration
dc.subjectTissue culture
dc.titleEnhancing in vitro micropropagation of Alternanthera reineckii Briq. using various light-emitting diodes, culture media and plant growth regulators
dc.title.alternativeEnhancing in vitro micropropagation of Alternanthera reineckii Briq using various light-emitting diodes, culture media and plant growth regulators
dc.typeArticle

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