A turn-off fluorescent sensor based on Schiff base incorporating diformyl phenol moiety for the detection of Cu2+ in aqueous solution at nanomolar level and its application in living cells

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dc.contributor.authorKeskinateş, Mukaddes
dc.contributor.authorAydın, Ziya
dc.contributor.authorYılmaz Altınok, Bahar
dc.contributor.authorBayrakçı, Mevlüt
dc.date.accessioned2025-01-12T17:08:55Z
dc.date.available2025-01-12T17:08:55Z
dc.date.issued2024
dc.departmentKMÜ, Kazım Karabekir Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.departmentKMÜ, Teknik Bilimler Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümü
dc.departmentKMÜ, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractGiven the critical role of trace copper ion in human health, its sensitive detection in environmental and biological samples is crucial. We, herein, designed and synthesized a diformyl phenol-based turn-off fluorescent sensor ((5-(tert-butyl)-2-hydroxy-1,3-phenylene)bis (methanylylidene)) bis (azanylylidene)) bis(4-hydroxy-2H-chromen-2-one) (MH-4)) for the spectrofluorometric detection of Cu2+ ion, which has sensitive and selective fluorescence quenching over other competitive metal ions. The efficiency and fast response of the sensor were confirmed by UV–Visible, fluorescence spectral changes, which may be due to the formation of coordination bond between MH-4 and Cu2+ ions. The binding stoichiometry of MH-4 with Cu2+ was examined with the help of UV–Vis and fluorescence titrations, and Job's plot, which gives a 1:1 coordination stoichiometry; this is further confirmed through MS studies. The binding constant of the complex was found to be 9.4 x 107 M?1. Then, the absorbance and fluorescence-based detection limits were calculated to define the sensitivity range of the proposed sensor and were found to be 69.30 nM and 37.40 nM, respectively. Finally, a live cell image study of MH-4 in L929 (murine aneuploid fibrosarcoma) cells was also performed to test the cell permeability of MH-4 and its effectiveness for selective detection of Cu2+ in living cells. © 2024 Elsevier B.V.
dc.description.sponsorshipKaramanoğlu Mehmetbey Üniversitesi, KMÜ
dc.identifier.citationKeskinates, M., Aydin, Z., Yilmaz Altinok, B., & Bayrakci, M. (2024). A turn-off fluorescent sensor based on Schiff base incorporating diformyl phenol moiety for the detection of Cu2+ in aqueous solution at nanomolar level and its application in living cells. Journal of Photochemistry & Photobiology, A: Chemistry, 455. https://doi.org/10.1016/j.jphotochem.2024.115765
dc.identifier.doi10.1016/j.jphotochem.2024.115765
dc.identifier.issn1010-6030
dc.identifier.scopus2-s2.0-85193244172
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jphotochem.2024.115765
dc.identifier.volume455
dc.indekslendigikaynakScopus
dc.institutionauthoridKeskinateş, Mukaddes/0000-0002-1799-7180
dc.institutionauthoridAydın, Ziya/0000-0001-8074-9510
dc.institutionauthoridYılmaz Altınok, Bahar/0000-0001-5645-7489
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Photochemistry and Photobiology A: Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20250111
dc.subjectChemosensor
dc.subjectCu<sup>2+</sup>
dc.subjectFluorescence
dc.subjectLive cell imaging
dc.subjectTurn-off
dc.titleA turn-off fluorescent sensor based on Schiff base incorporating diformyl phenol moiety for the detection of Cu2+ in aqueous solution at nanomolar level and its application in living cells
dc.typeArticle

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