Label-free G-Quadruplex aptamer and Thioflavin-T based turn-off fluorescent detection of ethanolamine

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dc.authorid0000-0002-8049-7867en_US
dc.contributor.authorBayraç, Abdullah Tahir
dc.contributor.authorAcar, Yasemin
dc.date.accessioned2019-12-06T21:15:00Z
dc.date.available2019-12-06T21:15:00Z
dc.date.issued2020
dc.departmentKMÜ, Mühendislik Fakültesi, Biyomühendislik Bölümüen_US
dc.descriptionWOS:000489191200004en_US
dc.description.abstractA label free aptasensor based on G-quadruplex forming ethanolamine aptamers and Thioflavin T was developed for the selective and sensitive detection of ethanolamine. The detection system consists of DNA aptamers with four GGG repeats forming a G-quadruplex that Thioflavin T can easily bind to causing high fluorescence emission intensity because of its fluorescence molecular rotor nature. In the presence of ethanolamine, binding of aptamer to its target induces conformational changes causing the separation of Thioflavin T from the complex with a dramatic decrease in fluorescence intensity of reaction mixture. This fluorescence aptasensor has a convenient sensitivity and selectivity with a detection limit of 641 mu M to measure ethanolamine directly in contaminated water samples in a wide linear range. Furthermore this method avoids all complicated modification and labelling steps and thus offers a simple, fast, and cost efficient solution for ethanolamine detection.en_US
dc.identifier.citationBayraç, A. T., Acar, Y. (2020). Label-free G-Quadruplex aptamer and Thioflavin-T based turn-off fluorescent detection of ethanolamine. Dyes and Pigments, 172.
dc.identifier.doi10.1016/j.dyepig.2019.107788
dc.identifier.issn0143-7208
dc.identifier.issn1873-3743
dc.identifier.scopus2-s2.0-85070617190
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1016/j.dyepig.2019.107788
dc.identifier.urihttps://hdl.handle.net/11492/2346
dc.identifier.volume172en_US
dc.identifier.wosWOS:000489191200004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorBayraç, Abdullah Tahir
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.journalDyes and Pigmentsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEthanolamineen_US
dc.subjectAptasensoren_US
dc.subjectG-Quadruplexen_US
dc.subjectFluorescence Molecular Rotoren_US
dc.titleLabel-free G-Quadruplex aptamer and Thioflavin-T based turn-off fluorescent detection of ethanolamineen_US
dc.typeArticle

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