Başarı, HasanYöntem, MustafaErci, FatihEsirgenler, Büsra2025-03-242025-03-242025Başarı, H., Yöntem, M., Erci, F., Esirgenler, B. (2025).Evaluation of synthesis variables on the antimicrobial and antioxidant efficacy of silver nanoparticles produced using hibiscus sabdariffa extract. Letters in Applied NanoBioScience,14(2).https://doi.org/10.33263/LIANBS142.057https://hdl.handle.net/11492/10705The increasing interest in biological synthesis techniques can be attributed to the need for non-toxic ways to produce nanoparticles. This study investigated the influence of different parameters on the synthesis of silver nanoparticles (AgNPs) using Hibiscus sabdariffa extract. Several analytical techniques were used to characterize the synthesized AgNPs. Furthermore, the antimicrobial and DPPH radical scavenging activities of the AgNPs were evaluated. XRD analysis validated the synthesis of pure nanosized crystalline silver nanoparticles. At room temperature, as the pH rised during the synthesis, the size of the nanoparticles decreased. Additionally, pH had the biggest impact on the distinctive qualities of AgNPs, while extract concentration, metal salt, and pH all affected the nanoparticles' zeta potential. The synthesized AgNPs showed antibacterial activity against Gram-positive and Gram-negative bacteria, with the greatest activity observed against Staphylococcus aureus. AgNPs have been shown to have greater antibacterial activity when synthesized at a pH of 7.5, as opposed to pH values of 8.5 and 10.5. To sum up, pH played a significant role in the synthesis of AgNPs and was essential for determining the biological activities of the particles. According to this study, it is possible to control nanoparticle synthesis parameters for applications in various industries, such as the food and medical sectors.enAntibacterial ActivityAntioxidant ActivityHibiscus SabdariffaSilver NanoparticlesSynthesis ParametersArticle142info:eu-repo/semantics/closedAccess10.33263/LIANBS142.057