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    CD19 Deficiency Leads to Dysregulation of Phosphoinositide 3-Kinase-Protein Kinase B (PI3K/AKT) and Nuclear Factor Kappa B (NF-κB) Pathways: Implications for B-Cell Maturation and Immune Function
    (Erciyes Üniv Sch Medıcine, 2025) Küçcüktürk, Serkan; Karaselek, Mehmet Ali; Duran, Tugce; Reisli, Ismail
    Objective: CD19 is a cellular receptor belonging to the immunoglobulin (Ig) superfamily and serves as a critical signaling component in B-cells differentiation and activation. This study investigates gene expression changes in the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) pathways in patients with CD19 deficiency. The role of CD19 in B-cell function was explored, along with its potential impact on these signaling pathways and the overall immune response. Materials and Methods: RNA samples were obtained from three patients diagnosed with CD19 deficiency, as well as heterozygous carriers and healthy controls. Gene expression profiles related to the PI3K/AKT axis and NF-kappa B pathway were analyzed using quantitative polymerase chain reaction (PCR). Results:The study revealed significant alterations in the expression of signaling pathway components, including PI3K, phosphoinositide-3-kinase regulatory subunit 1 (p85),TNF receptor-associated factor 2 (TRAF2), forkhead box O1 (FOXO1), and NF-kappa B, in CD19deficient patients. While PI3K and NF-kappa B expression were significantly downregulated in these patients, CD19, p85, FOXO1, and TRAF2 expression were markedly upregulated. These changes suggest impaired B-cell function, leading to weakened immune system responses. Conclusion: CD19 deficiency disrupts B-cell receptor signaling, resulting in significant alterations in the PI3K/AKT axis and NF-kappa B pathways. These disruptions impair B-cell maturation and survival, ultimately leading to compromised immune responses. This study provides the first detailed molecular insights into the effects of CD19 deficiency, enhancing our understanding of how these signaling pathways regulate immune function.
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    Effect of intensive training on immune system cells in elite female weightlifters
    (Assoc Medica Brasileira, 2024) Karaselek, Mehmet Ali; Kuccukturk, Serkan; Duran, Tugce
    OBJECTIVE: This study aimed to investigate the effects of intense weightlifting training on lymphocyte and natural killer cell subgroups, which are the major cells of the immune system, in elite female weightlifters.METHODS: A total of 20 elite female weightlifters were evaluated using flow cytometry before training (pre-T), immediately after training (post-T), and after a 120-min rest period (rest-T).RESULTS: Post-T and rest-T showed significant decreases in helper T (Th) and cytotoxic T compared with pre-T (p=0.045, p<0.001 and p=0.05, p<0.001, respectively). B and natural killer cells were higher in post-T and rest-T than in pre-T. The increase in B cells was significant in pre-T/rest-T (p<0.001) but not in pre-T/post-T (p=0.122). Intense training significantly increased natural killer cells in both post-T and rest-T (p<0.001). CD56brightand CD56dim natural killer cell subgroups were significantly lower in post-T and rest-T than in pre-T (p=0.005, p=0.006 and p<0.001, p=0.004, respectively).CONCLUSION: This study shows that intense weightlifting alters peripheral lymphocyte and natural killer subgroup ratios, being the first investigation in this field.
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    Effect of intensive training on immune system cells in elite female weightlifters
    (Assoc Medica Brasileira, 2024) Karaselek, Mehmet Ali; Kuccukturk, Serkan; Duran, Tugce
    OBJECTIVE: This study aimed to investigate the effects of intense weightlifting training on lymphocyte and natural killer cell subgroups, which are the major cells of the immune system, in elite female weightlifters. METHODS: A total of 20 elite female weightlifters were evaluated using flow cytometry before training (pre -T), immediately after training (post -T), and after a 120 -min rest period (rest -T). RESULTS: Post -T and rest -T showed significant decreases in helper T (Th) and cytotoxic T compared with pre -T (p=0.045, p<0.001 and p=0.05, p<0.001, respectively). B and natural killer cells were higher in post -T and rest -T than in pre -T. The increase in B cells was significant in pre-T/rest-T (p<0.001) but not in pre-T/post-T (p=0.122). Intense training significantly increased natural killer cells in both post -T and rest -T (p<0.001). CD56(bright) and CD56(dim) natural killer cell subgroups were significantly lower in post -T and rest -T than in pre -T (p=0.005, p=0.006 and p<0.001, p=0.004, respectively). CONCLUSION: This study shows that intense weightlifting alters peripheral lymphocyte and natural killer subgroup ratios, being the first investigation in this field.
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    Evaluation of transcription factors and cytokine expressions of T-cell subsets in CD19 deficiency and their possible relationship with autoimmune disease
    (Wiley, 2023) Kueccuektuerk, Serkan; Karaselek, Mehmet Ali; Duran, Tugce; Reisli, Ismail
    CD19 deficiency is a rare, predominantly antibody deficiency, and there are few studies showing that it can be seen in autoimmune diseases. The aim of study was evaluated to transcription factor and cytokine expressions of helper T (Th)-cell subsets in CD19 deficiency and the possible mechanism role of this factor expression in autoimmune disease. Transcription factor and cytokine expressions of Th1, Th2, Th17, and regulatory T (Treg) cells were investigated by real-time polymerase chain reaction (qPCR) method. In the study, in the patient/control comparison, transcription factor and cytokine expressions of Th1 (T-bet, STAT1, and STAT4) were found to be significantly downregulated, but IFN-gamma was significantly upregulated in patients. Th2 factor GATA3, STAT6, IL-4, and IL-5 were significantly downregulated. For Th17, ROR gamma t was downregulated while IL-22 was upregulated. In the heterozygous/control comparison, there was no significant change in gene expressions other than IL-5. T-bet, STAT1, GATA3, IL-4, ROR gamma t, FoxP3, and TGF-beta were significantly downregulated in the patient/heterozygous comparison. It was revealed for the first time that the expression of the transcription factors and cytokines in CD19 deficiency. These findings might be showing the predominance of Th1 factors and suppressed Treg factors which could be related with autoimmunity in CD19 deficiency.
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    Investigation of the Antitumour and Anticancer Effects of Neferine by Inducing Stress of Endoplasmic Reticulum in Human Cervical Cancer-Derived Cells (HeLa)
    (Kamla-Raj Enterprises, 2023) Sahin, Gozde; Duran, Tugce; Kocak, Nadir; Bayramoglu, Denizhan; Canday, Mujde; Yurtkal, Asli
    The leading prevalent reason of cancer demise in women is cervical cancer. The endoplasmic reticulum (ER) is necessary in order to provide homeostasis of the cell as well could have a close relation with cancer. Neferine may exert anticancer effects by inducing apoptosis and stress of ER. The researchers observed neferine's antitumoural efficacy in human cervical cancer derived cells (HeLa) via ER stress. Neferine was applied to the HeLa cells, and a viability test was performed by the methyl thiazolyl tetrazolium proliferation (MTT) assay. Analysis of MTT assay demonstrated that neferine effectively blocked HeLa cells development in a dose-dependently. Neferine may promote ER stress and enhance anticancer efficacy in HeLa cells. Based on Western blot and quantitative real-time polymerase chain reaction (qPCR) analyses, the researchers found that neferine inhibited cervical cancer cells via the ER stress pathway. The results indicate that neferine is a potent anticancer therapeutic candidate.
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    Molecular investigation of the effect of weightlifting training on helper T cell subsets in female weightlifting athletes: an immune profiling panel
    (Edizioni Minerva Medica, 2023) Duran, Tugce; Karaselek, Mehmet A.; Kuccukturk, Serkan
    BACKGROUND: In this study, it was aimed to molecularly investigate the changes in the helper T (Th) cell subgroups of intense weightlifting training. For this purpose, transcription factor and cytokine expressions of Th1, Th2, Th17, and regulator T cell (Treg) cells were evaluated.METHODS: Eight elite weightlifting athletes were included in the study. Within the scope of the study, transcription factor and cytokine expressions of Th cell subgroups were evaluated by real-time polymerase chain reaction (qPCR) method before training, after intense training with 90-100% capacity, and after 120 minutes of rest from training.RESULTS: As a result of the study, when the pre-training and post-training expressions were compared, an increase in Th1 and Th2 cell factors and a decrease in Th17 and Treg cell-related expressions were detected. These changes were statistically significant (P<0.05). The changes in expressions after training and after 120 minutes of rest were not statistically significant (P>0.05).CONCLUSIONS: Changes have been detected in Th cell subgroups due to intense weightlifting training, and these changes are the first study conducted with female weightlifters.