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  • Küçük Resim
    Öğe
    Characterization of silver nanoparticles synthesized using hypericum perforatum L. and their effects on staphylococcus aureus
    (Wiley, 2025) Şaşmaz, Canan Sevinç; Erci, Fatih; Torlak, Emrah; Yöntem, Mustafa
    This study investigates the synthesis of silver nanoparticles (AgNPs) using Hypericum perforatum L. and evaluates their antibacterial and antibiofilm activities against Staphylococcus aureus. The synthesized AgNPs were characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). UV-Vis spectroscopy showed a maximum absorption peak at 448 nm, which indicates that nanoparticles have been formed successfully. TEM analysis showed that the AgNPs were spherical, with an average size of 35 ± 2.7 nm. FTIR confirmed the presence of functional groups on the surface of AgNP that may be contributing to its biological activity. The AgNPs exhibited significant antibacterial activity, with a minimum inhibitory concentration (MIC) of 75 μg/mL and an inhibition zone of 13 ± 0.13 mm at this concentration. They were also highly effective in inhibiting biofilm formation even at a concentration of 25 μg/mL, reducing biofilm formation by 47.25% ± 3.51%. At increased concentrations, nanoparticles have been shown to compromise bacterial membranes, leading to significant membrane disruption. This disruption subsequently results in a reduction of cellular respiration, with observed decreases of approximately twofold when compared to controls. Additionally, nanoparticles facilitate the production of superoxide anions, which can rise by about threefold, consequently enhancing the overall effectiveness of bacterial inactivation. Field emission scanning electron microscopy (FE-SEM) revealed structural damage to bacterial cells treated with AgNPs, supporting their antimicrobial effects. These findings suggest that AgNPs synthesized from H. perforatum could serve as effective antimicrobial agents against S. aureus. Their ability to disrupt bacterial cell membranes, inhibit respiration, and induce oxidative stress makes them promising candidates for antimicrobial and antibiofilm applications, particularly given the increasing concern over bacterial resistance to conventional antibiotics.
  • [ X ]
    Öğe
    Evaluation of synthesis variables on the antimicrobial and antioxidant efficacy of silver nanoparticles produced using hibiscus sabdariffa extract
    (AMG Transcend Association, 2025) Başarı, Hasan; Yöntem, Mustafa; Erci, Fatih; Esirgenler, Büşra
    The increasing interest in biological synthesis techniques can be attributed to the need for non-toxic ways to produce nanoparticles. This study investigated the influence of different parameters on the synthesis of silver nanoparticles (AgNPs) using Hibiscus sabdariffa extract. Several analytical techniques were used to characterize the synthesized AgNPs. Furthermore, the antimicrobial and DPPH radical scavenging activities of the AgNPs were evaluated. XRD analysis validated the synthesis of pure nanosized crystalline silver nanoparticles. At room temperature, as the pH rised during the synthesis, the size of the nanoparticles decreased. Additionally, pH had the biggest impact on the distinctive qualities of AgNPs, while extract concentration, metal salt, and pH all affected the nanoparticles' zeta potential. The synthesized AgNPs showed antibacterial activity against Gram-positive and Gram-negative bacteria, with the greatest activity observed against Staphylococcus aureus. AgNPs have been shown to have greater antibacterial activity when synthesized at a pH of 7.5, as opposed to pH values of 8.5 and 10.5. To sum up, pH played a significant role in the synthesis of AgNPs and was essential for determining the biological activities of the particles. According to this study, it is possible to control nanoparticle synthesis parameters for applications in various industries, such as the food and medical sectors.
  • [ X ]
    Öğe
    Evaluation of synthesis variables on the antimicrobial and antioxidant efficacy of silver nanoparticles produced using hibiscus sabdariffa extract
    (AMG Transcend Association, 2025) Başarı, Hasan; Yöntem, Mustafa; Erci, Fatih; Esirgenler, Büsra
    The increasing interest in biological synthesis techniques can be attributed to the need for non-toxic ways to produce nanoparticles. This study investigated the influence of different parameters on the synthesis of silver nanoparticles (AgNPs) using Hibiscus sabdariffa extract. Several analytical techniques were used to characterize the synthesized AgNPs. Furthermore, the antimicrobial and DPPH radical scavenging activities of the AgNPs were evaluated. XRD analysis validated the synthesis of pure nanosized crystalline silver nanoparticles. At room temperature, as the pH rised during the synthesis, the size of the nanoparticles decreased. Additionally, pH had the biggest impact on the distinctive qualities of AgNPs, while extract concentration, metal salt, and pH all affected the nanoparticles' zeta potential. The synthesized AgNPs showed antibacterial activity against Gram-positive and Gram-negative bacteria, with the greatest activity observed against Staphylococcus aureus. AgNPs have been shown to have greater antibacterial activity when synthesized at a pH of 7.5, as opposed to pH values of 8.5 and 10.5. To sum up, pH played a significant role in the synthesis of AgNPs and was essential for determining the biological activities of the particles. According to this study, it is possible to control nanoparticle synthesis parameters for applications in various industries, such as the food and medical sectors.