Yazar "Köksoy, Hale" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Different approach on our cells and the use of CRISPR/Cas 9 technology: Super gene'ethic
    (2023) Köksoy, Hale; Qadri, Mohammed S.; Karakaya, Serap; Bacak, Pelin; S.Yüksel, Elif; Koçer, Şengül
    CRISPR/Cas9, a revolutionary genome editing tool derived from a bacterial system, has transformed molecular biology research, enabling precise and versatile modifications to the genome. This study surveyed 300 participants, including instructors, second-year medical students, and patient relatives from Selcuk University Faculty of Medicine, to assess their knowledge, attitudes, and ethical perspectives on gene editing. Methods: A comprehensive survey was conducted, and the participants' demographics were correlated with their responses. Statistical analysis was performed using SPSS 22.0. Results: The survey revealed significant differences (p<0.05) in attitudes based on gender, age, and participation groups. The age group of 18-29 exhibited the highest participation rate (57.3%), while the age group of 60-65 had the lowest (1%). Approximately 50.3% of participants reported no prior knowledge of CRISPR/Cas9, and 64.3% were university graduates. Obtaining approval from ethics committees was considered crucial by 151 respondents for commencing CRISPR/Cas9 research. Interestingly, 63.3% showed eagerness for genetically modified plants and animals, while only 9% expressed interest in studies related to eternal youth. Additionally, 88% found gene editing studies conflicting with their beliefs. Conclusions: CRISPR/Cas9 holds great promise for biomedicine and healthcare but necessitates enhanced public awareness and informed decision-making. The study highlights the importance of promoting informed discussions on the ethical implications of CRISPR/Cas9 to address the concerns and perceptions of various demographics. These valuable insights contribute to the ongoing discourse on the impact of CRISPR/Cas9 on society.
  • Küçük Resim
    Öğe
    Evaluation of ın vitro antimicrobial activity of epigallocatechin gallate (egcg) and green tea (camellia sinensis) oil on various pathogens
    (Selcuk University, 29 February 2024) Köksoy, Hale; Rağbetli, Cennet
    Epigallocatechin gallate (EGCG), the polyphenolic component of Camellia sinensis catechins, and Camellia sinensis extract have broad antimicrobial activity. This study aimed to investigate the invitro antibacterial and antifungal activity of the Epigallocatechin gallate and Camellia sinensis extract. Method: The present study tested the antibacterial and antifungal activity of Epigallocatechin gallate and Camellia sinensis extract against some gram-negative, gram-positive, and fungal isolates of American Type Culture Collection (ATCC). The minimum inhibitory concentration (MIC) of Epigallocatechin gallate (EGCG) and Camellia sinensis extract were determined for each test microorganism. In general, EGCG and Camellia sinensis extract results were found compatible. Epigallocatechin gallate and Camellia sinensis extract exhibited antibacterial and antifungal activity against all test organisms. MIC was determined visually after 16-20 hours of incubation at 37°C according to broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI). Result: The Camellia sinensis extract and EGCG MIC for gram-positive bacteria was found lower than the MIC for gram-negative bacteria and the Camellia sinensis extract MIC for C. albicans was higher than the EGCG MIC. Conclusions: However, EGCG was more effective on C. albicans isolate than Camellia sinensis extract. Camellia sinensis extract Epigallocatechin gallate and Camellia sinensis extract may be a promising source of antibacterial and antifungal for further studies.