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Öğe The effect of curcumin on penile fibrotic plaque in rats with experimental peyronie’s disease(2023) Ulubay, Mahmut; Kaya, Ayşenur; Kaplan, Süleyman; Kaplan, Arife Ahsen; Tüfekçi, Kıymet Kübra; Akdeniz, EkremObjective: No effective medical approach for the treatment of Peyronie’s disease (PD) has to date been described. This study was intended to evaluate the antifibrotic, antioxidant, and anti-inflammatory effects of curcumin on fibrotic tissue in the tunica albuginea (TA) in a rat model of PD. Materials and Methods: Twenty-four male Sprague Dawley rats aged 10 months were randomized into three groups (n = 8 in each). No PD model was induced in the control group. The PD+saline (PD+Ps) group received fibrin injection, followed two weeks later by saline administration by oral gavage for 14 days. The PD+Curcumin (PD+Cur) group received fibrin injection into the TA followed two weeks later by curcumin administration by oral gavage for 14 days. At the end of the experiment, fibrotic activity was evaluated using stereological and histopathological methods. Transforming growth factor-?1 (TGF-?1), one of the most fibrogenic cytokines, was evaluated using immunohistochemistry with an anti-TGF-?1 rabbit monoclonal antibody. Results: Stereological analysis revealed significantly greater Peyronie-like plaque areas in the TA in the PD+Ps group than in the control and PD+Cur groups (p<0.0001). No significant difference was observed between the control and PD+Cur groups (p=0.35). The PD+Ps group exhibited strong TGF?1 immunoreactivity with increased expression in the collagenous connective tissues and fibroblasts around the TA. Conclusion: Curcumin reduced fibrotic tissue in the TA and may represent a novel therapeutic option in the treatment of PD.Öğe The effects of Garcinia kola and curcumin on the dorsal root ganglion of the diabetic rat after peripheral nerve transection injury(Elsevier B.V., 2024) Aktaş, Abit; Yiğit, Funda; Delibaş, Burcu; Kaplan, Arife Ahsen; Hamour, Hala Mahgoub; Kaya, AyşenurObjective: To test the protective effects of Garcinia kola and curcumin on the ganglion tissues of diabetic rats following the use of autologous vein graft in peripheral nerve transection injury. Methods: The sciatic nerve on the right side was transected, and anastomosis was performed between the proximal and distal ends using an autologous vein graft. Curcumin and Garcinia kola seed extract were administered daily by oral gavage. The ganglion tissues were harvested after a 90-day waiting period. Sensory neurons in the dorsal root ganglion at the L4 and L5 levels were used for stereological evaluations. Mean sensory neuron numbers were analyzed using a stereological technique. The size of the light and dark neurons was also estimated, and ultrastructural and immunohistochemical evaluations were performed. Results: A statistically significant difference in sensory neuron numbers was observed between the groups with and without Garcinia kola and curcumin applications. The immunohistochemical results showed that the s-100 protein is expressed selectively between cell types. Conclusion: The results of this study show that curcumin and Garicinia kola prevented sensory neuron loss in diabetic rats following transection injury to the sciatic nerve.Öğe Stereological analysis of the gastrointestinal tract(Nova Science Publishers, Inc., 2024) Ahmed, Mohamed Kheir; Kaya, Ayşenur; Kaplan, Arife Ahsen; Deniz, Ömür Gülsüm; Önger, Mehmet Emin; Kaplan, SüleymanThis chapter provides an overview of the stereology, normal anatomy, and histology of the rat gastrointestinal tract (GIT). In addition to staining and tissue processing, various sampling protocols and stereological techniques that can be used to estimate the volume and/or volume fraction of any GIT layers, such as the Cavalieri principle, are also discussed. A number of methods are also available for calculating the numbers of any GIT cells. This chapter discusses physical and optical disectors, as well as fractionators, and how these can be applied in the GIT. Images have been added to simplify the discussion. © 2024 Nova Science Publishers, Inc. All rights reserved.Öğe Uterus stereology(Nova Science Publishers, Inc., 2024) Kaya, Ayşenur; Altun, Gamze; Tüfekçi, Kıymet Kübra; Kaplan, Arife Ahsen; Kipanyula, Maulilio J.; Kaplan, SüleymanInfertility is a growing global health problem with important socio-psychological consequences. The main causes of infertility are the pathogenesis of the uterine mucosa and the uterus being unready for implantation as a result of decidualization. Despite the critical importance of the uterus for women's health and fertility, and embryo development, relatively limited information is available concerning the hormonal, cellular, and molecular mechanisms in its pathogenesis. Morphoquantitative assessments are widely used by researchers to explain the underlying causes of infertility. Quantitative evaluations provide more descriptive and precise information when observing structural changes in tissue under different conditions and comparing these between different groups. Design-based stereology provides an accurate and unbiased estimation of stereological parameters, avoiding sources of bias caused by the methodology employed. It is therefore important to use stereological methods when considering a quantitative morphological evaluation of biological objects. This chapter describes the importance of developing and disseminating the use of stereological techniques in uterine research and is intended to explain the modern stereological techniques applied to uterine tissue in methodological terms. © 2024 Nova Science Publishers, Inc. All rights reserved.
