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  • Küçük Resim
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    Folic-acid-modified conducting polymer: electrochemical detection of the cell attachment
    (Wiley-VCH Verlag GMBH, 2016) Azak, Hacer; Barlas, Fırat Barış; Yıldız, Hüseyin Bekir; Güleç, Kadri; Demir, Bilal; Demirkol, Dilek Odacı; Timur, Suna
    Here, postfunctionalization and bioapplication of a pi-conjugated polymer named 4-[4H-dithieno(3,2-b:2',3'-d)pyrrol-4-yl]aniline (DTP-aryl-NH2) are reported, which is successfully synthesized via electropolymerization onto the glassy carbon electrode. Folic acid (FA) is used to modify the amino functional polymer via N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide chemistry for the further steps. The selective adhesion of folate receptor positive cells on the surface is followed by the electrochemical methods. Cyclic voltammetry and electrochemical impedance spectroscopy have been used to characterize stepwise modification of the electroactive surface. After optimization studies such as scan rate during the polymer deposition, FA amount for the efficient surface targeting, incubation time with the cells etc., analytical characterization is carried out. The surface morphologies at each step are imaged by using fluorescence microscopy.
  • Küçük Resim
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    Synthesis of Amine Functionalized Naphthalene Containing Conducting Polymer as a Matrix for Biomolecule Immobilization
    (Yayıncı Yok, 2013) Azak, Hacer; Yıldız, Hüseyin Bekir; Demirkol, Dilek Odacı; Can, Ümmügülsüm; Talaz, Oktay; Timur, Suna
    DTP (polydithienopyrroles) have been investigated as a new unit in conjugated materials because of its planar structure, extended -conjugation across the fused rings and N-H bond. Generally Nsubstituted dithione [3,2-b:2’,3’-d] pyroles (DTPs) are precursors for conducting polymers[1].
  • Küçük Resim
    Öğe
    Synthesis of an amine-functionalized naphthalene-containing conducting polymer as a matrix for biomolecule immobilization
    (Royal Soc Chemistry, 2013) Azak, Hacer; Güler, Emine; Can, Ümmügülsüm; Demirkol, Dilek Odacı; Yıldız, Huseyin Bekir; Talaz, Oktay; Timur, Suna
    N-functionalized dithienopyrroles (DTP-NH2) were synthesized and electropolymerized onto a graphite electrode as a novel conducting polymer matrix for biomolecule immobilization. H-1-NMR and C-13-NMR were utilized to investigate the characteristics of the monomer. After that, glucose oxidase (GOx) was immobilized onto the amino-functionalised matrix by means of glutaraldehyde. The surface morphologies of both DTP-NH2 and DTP-NH2-GOx were visualised by using SEM and fluorescence microscopy. The chronoamperometric signals of the electrochemical DTP-NH2-GOx biosensors were measured by monitoring the O-2 consumption during an enzymatic reaction in the presence of glucose at -0.7 V. After the optimization of the pH and scan number of the polymer deposition in batch mode, the DTP-NH2-GOx biosensor was also tested in Flow Injection Analysis (FIA) mode. The DTP-NH2-GOx biosensors had a very good linearity between 0.05 and 1.0 mM, and between 0.1 and 2.5 mM for glucose in batch and FIA modes, respectively. Finally, it was applied for glucose analysis in real samples where commercial glucose kits were used as the reference method to verify the data obtained with the proposed biosensor.