Entrapment Of Tyrosinase In A Redox Polymer
| dc.authorid | 0000-0003-1123-8797 | en_US |
| dc.contributor.author | Yıldız, Hüseyin Bekir | |
| dc.contributor.author | Castillo, Jaime | |
| dc.contributor.author | Guschin, Dmitrii A. | |
| dc.contributor.author | Schuhmann, Wolfgang | |
| dc.contributor.author | Toppare, Levent | |
| dc.date.accessioned | 2014-10-23T12:32:18Z | |
| dc.date.available | 2014-10-23T12:32:18Z | |
| dc.date.issued | 2012 | |
| dc.department | KMÜ, Kamil Özdağ Fen Fakültesi, Kimya Bölümü | en_US |
| dc.description.abstract | An amperometric biosensor for the detection of phenolic compounds was developed based on the immobilization of tyrosinase within an Os-complex functionalized electrodeposition polymer. Integration of tyrosinase within the redox polymer assures efficient catechol recycling between the enzyme and the polymer bound redox sites. The non-manual immobilization procedure improves the reproducibility of fabrication process, greatly reduces the desorption of the enzyme from the immobilization layer, and, most importantly prevents fast inactivation of the enzyme by its substrate due to fast redox cycling. A two-layer sensor architecture was developed involving ascorbic acid oxidase entrapped within an electrodeposition polymer in a second layer on top of the redox polymer=tyrosinase layer. Using this sensor architecture it was possible to eliminate the current interference arising from direct ascorbate oxidation up to a concentration of 630 mM ascorbic acid. The effects of the polymer thickness, the enzyme=polymer ratio, and the applied potential were evaluated with respect to optimal sensor properties. The sensitivity of the optimized sensors for catechol was 6.1 nA mM_1 with a detection limit of 10 nM, and for phenol 0.15 nA mM_1 with a detection limit of 100 nM. | en_US |
| dc.identifier.citation | Yıldız, H. Bekir...(ve ark.)."“Entrapment Of Tyrosinase In A Redox Polymer”, 25th The International Symposium on Polymer Analysis and Characterization (ISPAC 2012), 11-13 Haziran 2012, Rolduc Abbey, Hollanda | en_US |
| dc.identifier.uri | https://hdl.handle.net/11492/931 | |
| dc.institutionauthor | Yıldız, Hüseyin Bekir | |
| dc.language.iso | en | |
| dc.publisher | ISPAC 2012 | en_US |
| dc.relation.publicationcategory | Konferans Öğesi - Uluslararası - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | Entrapment Of Tyrosinase In A Redox Polymer | en_US |
| dc.type | Conference Object |
