Cytotoxicity of Usnea longissima Ach. extracts and its secondary metabolite, usnic acid on different cells

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dc.authorid0000-0002-9636-2596
dc.contributor.authorEngin, Tubanur Aslan
dc.contributor.authorİnan, Benan
dc.contributor.authorEmsen, Buğrahan
dc.contributor.authorÖztürk, Rabia Yılmaz
dc.contributor.authorÖzçimen, Didem
dc.contributor.authorKoç, Rabia Cakir
dc.date.accessioned2024-01-22T12:23:00Z
dc.date.available2024-01-22T12:23:00Z
dc.date.issued2023
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe biological activities of lichens, known as organisms based on a symbiotic relationship, are attracting more and more attention in traditional medicine and modern drug research. Lichens can possess various pharmacological effects such as antimicrobial, antioxidant, antitumor, anti-inflammatory, and many others due to the bioactive compounds they contain. In the present study, Usnea longissima Ach. and its secondary metabolite, usnic acid on human gastric adenocarcinoma cells (AGS), human colorectal adenocarcinoma cells (Caco-2), and mouse fibroblasts (NIH/3T3) were investigated. In this context, methanol and water extracts from U. longissima were obtained by Soxhlet extractor. The characterization of usnic acid was carried out by fourier transform infrared spectroscopy (FTIR). The cytotoxic activities of the extracts and the metabolite on cells were determined by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) analysis. Considering the median inhibitory concentration (IC50) values, the application with the greater effect on AGS and NIH/3T3 cells was the methanol extract (373.17 µg/ml and 318.81 µg/ml, respectively). Considering the Caco-2 cells, it was determined that the water extract had the lowest IC50 value (230.05 µg/ml). The high cytotoxic activity of usnic acid on cancer cells (AGS; IC50: 395.03 µg/ml and Caco-2; IC50: 462.35 µg/ml) compared to normal cell (NIH/3T3; IC50: 472.41 µg/ml) was noted. As a result, it has been revealed that methanol and water extracts of U. longissima, especially usnic acid, are products that can be used within the scope of complementary therapy.en_US
dc.identifier.citationAslan Engin T, Emsen B, Yılmaz Öztürk R, Çakır Koç R, İnan B, Özçimen D (2023). Cytotoxicity of Usnea longissima Ach. extracts and its secondary metabolite, usnic acid on different cells. Anatolian Journal of Botany 7(2): 140-145.
dc.identifier.doi10.30616/ajb.1343823
dc.identifier.endpage145en_US
dc.identifier.issn2602-2818
dc.identifier.issue2en_US
dc.identifier.startpage140en_US
dc.identifier.trdizinid1208406
dc.identifier.urihttps://doi.org/10.30616/ajb.1343823
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1208406
dc.identifier.urihttps://hdl.handle.net/11492/8233
dc.identifier.volume7en_US
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorEmsen, Buğrahan
dc.language.isoen
dc.relation.ispartofAnatolian Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleCytotoxicity of Usnea longissima Ach. extracts and its secondary metabolite, usnic acid on different cellsen_US
dc.typeArticle

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