Purification strategies of Scytalidium thermophilum xylanase

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dc.authorid0000-0002-5689-8521en_US
dc.contributor.authorSutay Kocabaş, Didem
dc.contributor.authorGüder, Sevgi
dc.contributor.authorÖzben, Neslihan
dc.contributor.authorTur, Eren
dc.date.accessioned2015-01-23T08:39:31Z
dc.date.available2015-01-23T08:39:31Z
dc.date.issued2014-06
dc.departmentKMÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractXylanase (E.C.3.2.1.8) is an enzyme which hydrolyses b-1,4 bonds in xylan backbone which consists of D-xylose molecules. Based on its function, xylanase is used in many industries. For animal feed, paper production and textile industry partially purified xylanase can be acceptable, but for bakery and beverage industry, chemical or pharmaceutical applications, high purity is generally required. In this study, xylanase was produced from a thermophilic fungus, Scytalidium thermophilum and purification strategies were developed for industrial and analytical purposes. In addition, within the cost reduction approach, corn cobs are used as carbon source in the culture medium which are produced millions of tons as agricultural waste each year and have very low economical value. Partial purification of xylanase was studied with ammonium sulphate precipitation (ASP), aqueous two phase system (ATPS) and ultrafiltration (UF). Precipitation with the usage of 50% (v/v) ammonium sulphate concentration gave the best results as 2.5 fold purification and 52% yield. TX-114 was employed at a concentration of 7% (v/v) for ATPS and 2.7 fold purification with 79% yield was obtained. Three-step UF technique was practiced using membranes with different MWCO values; which were 100, 30 and 10 kDa, sequentially. As the result of this technique, 4.3 fold purification with 25% yield was attained. With these results, partially purified xylanase can be presented as an ingredient for industrial applications. For analytical purification of enzymeen_US
dc.identifier.citationSutay Kocabaş...(ve ark.)"Purification strategies of Scytalidium thermophilum xylanase",International Enzymes for Biocatalysis Workshop,2014.en_US
dc.identifier.urihttps://hdl.handle.net/11492/1045
dc.institutionauthorSutay Kocabaş, Didem
dc.institutionauthorGüder, Sevgi
dc.institutionauthorÖzben, Neslihan
dc.language.isoen
dc.publisherYayıncı Yoken_US
dc.relation.journalInternational Enzymes for Biocatalysis Workshopen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectXylanaseen_US
dc.subjectScytalidium thermophilumen_US
dc.subjectPurificationen_US
dc.subjectColumn hromatographyen_US
dc.titlePurification strategies of Scytalidium thermophilum xylanaseen_US
dc.typeConference Object

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