In vitro evaluation of cytotoxic, oxidative, genotoxic, and apoptotic activities of physodic acid from Pseudevernia furfuracea in HepG2 and THLE2 cells

Basit Öğe Kaydı
dc.authorid0000-0002-9636-2596en_US
dc.authorid0000-0002-6422-1203en_US
dc.authorid0000-0002-6855-0645en_US
dc.contributor.authorEmsen, Buğrahan
dc.contributor.authorSadi, Gökhan
dc.contributor.authorBostancı, Aykut
dc.contributor.authorAslan, Ali
dc.date.accessioned2020-12-31T11:14:26Z
dc.date.available2020-12-31T11:14:26Z
dc.date.issued2020en_US
dc.departmentKMÜ, Kamil Özdağ Fen Fakültesi, Biyoloji Bölümüen_US
dc.descriptionWOS:000596181000001en_US
dc.description.abstractIn the present study, cytotoxic (apoptotic and necrotic), antioxidant, prooxidant, genotoxic, and apoptosis-related genes expression modulatory effects of physodic acid (PA) isolated from Pseudevernia furfuracea on cancerous (HepG2) and healthy (THLE2) human liver cells were evaluated. Cytotoxic effects PA obtained from P. furfuracea on HepG2 and THLE2 cells were tested via MTT and LDH analyses. In order to determine genotoxic effect of PA, 8-OH-dG levels in the cells were measured. In addition, apoptotic or necrotic effects of PA on the hepatic cells were determined by qRT-PCR analysis. Cytotoxicity analyses revealed the cytotoxic effect of PA on HepG2 cells, which is much higher than THLE2 cells. Gene expression studies demonstrated the apoptotic effects of PA on HepG2 cells through both extrinsic and intrinsic pathways. PA increased the antioxidant capacity of THLE2 cells with increasing concentrations, which describes its antioxidant power. Additionally, none of the tested levels of PA caused oxidative DNA damage on THLE2 cells, but its high concentration increased genetic damages on HepG2. Taken together, P. furfuracea lichen and its metabolite, PA, would be a promising alternative for complementary treatments against liver cancer.en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) [grant number 117Z632]en_US
dc.identifier.citationEmsen,B., Sadi,G., Bostancı, A., Aslan, A.(2020). In vitro evaluation of cytotoxic, oxidative, genotoxic, and apoptotic activities of physodic acid from Pseudevernia furfuracea in HepG2 and THLE2 cells. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, doi: 10.1080/11263504.2020.1852329en_US
dc.identifier.doi10.1080/11263504.2020.1852329
dc.identifier.issn1126-3504
dc.identifier.issn1724-5575
dc.identifier.scopus2-s2.0-85097133987
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1080/11263504.2020.1852329
dc.identifier.urihttps://hdl.handle.net/11492/3760
dc.identifier.wosWOS:000596181000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorEmsen, Buğrahan
dc.institutionauthorSadi, Gökhan
dc.institutionauthorBostancı, Aykut
dc.language.isoen
dc.publisherTaylor and Francisen_US
dc.relation.journalPlant Biosystems - An International Journal Dealing with all Aspects of Plant Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak117Z632
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject8-Hydroxy-2&#8242en_US
dc.subjectDeoxyguanosineen_US
dc.subjectLactate Dehydrogenaseen_US
dc.subjectLichenen_US
dc.subjectTotal Antioxidant Capacityen_US
dc.subjectTotal Oxidative Stressen_US
dc.titleIn vitro evaluation of cytotoxic, oxidative, genotoxic, and apoptotic activities of physodic acid from Pseudevernia furfuracea in HepG2 and THLE2 cellsen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Emsen, Bugrahan 2020.pdf
Boyut:
1.98 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin /Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Kamil Özdağ Fen Fakültesi, Biyoloji Bölümü, Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu