Enzyme immobilization in a photosensitive conducting polymer bearing azobenzene in the main chain

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dc.contributor.authorAk, Metin
dc.contributor.authorYildiz, Hüseyin Bekir
dc.contributor.authorToppare, Levent
dc.date.accessioned2025-01-12T17:08:44Z
dc.date.available2025-01-12T17:08:44Z
dc.date.issued2014
dc.departmentKaramanoğlu Mehmetbey Üniversitesi
dc.description.abstractA new photosensitive and thermosensitive monomer, namely bis(4-(3-thienyl ethylene)-oxycarbonyl)diazobenzene (TDAZO), was synthesized. The photochemical and thermal cis-trans isomerization of the monomer has been investigated. The rate constants of the photoisomerization of TDAZO in ACN and DCM were 0.195 and 0.308 min-1, respectively. For spectroelectrochemical investigation and enzyme immobilization application, TDAZO copolymerized with thiophene and pyrrole. Electrochemical and spectroelectrochemical properties of P(TDAZO-co-Th) were investigated and invertase was immobilized in P(TDAZO-co-Py) copolymer. Immobilization of enzymes was carried out by the entrapment of the enzyme in conducting polymer matrices during electrochemical polymerization of pyrrole through thiophene moieties of the TDAZO. Optimum conditions for this electrode, such as pH, temperature, kinetic parameters (K m and V max) and operational stability were investigated. Kinetic parameters invertase-immobilized in copolymer were smaller than free enzyme. The optimum operational temperature was 10°C higher for immobilized enzyme than that of the free enzyme. Due to strong interaction between enzyme and diazo group in the polymer main chain, thermal, pH and operational stability of enzyme has been enhanced. © 2014 Springer-Verlag Berlin Heidelberg.
dc.identifier.doi10.1007/s00289-014-1157-7
dc.identifier.endpage1841
dc.identifier.issn0170-0839
dc.identifier.issue7
dc.identifier.scopus2-s2.0-84903397040
dc.identifier.scopusqualityQ1
dc.identifier.startpage1827
dc.identifier.urihttps://hdl.handle.net/11492/9003
dc.identifier.volume71
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Verlag
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20250111
dc.subjectConducting polymer
dc.subjectEnzyme immobilization
dc.subjectInvertase
dc.subjectPhotosensitive azobenzene
dc.titleEnzyme immobilization in a photosensitive conducting polymer bearing azobenzene in the main chain
dc.typeArticle

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