Assessment of spatially offset Raman spectroscopy to detect differences in bone matrix quality

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dc.authoridGautam, Rekha/0000-0002-1176-8491
dc.authoridNyman, Jeffry/0000-0001-7403-7605
dc.authoridAhmed, Rafay/0000-0002-0164-8525
dc.contributor.authorGautam, Rekha
dc.contributor.authorAhmed, Rafay
dc.contributor.authorHaugen, Ezekiel
dc.contributor.authorUnal, Mustafa
dc.contributor.authorFitzgerald, Sean
dc.contributor.authorUppuganti, Sasidhar
dc.date.accessioned2024-01-22T12:22:17Z
dc.date.available2024-01-22T12:22:17Z
dc.date.issued2023
dc.departmentKMÜen_US
dc.description.abstractSince spatially offset Raman spectroscopy (SORS) can acquire biochemical measurements of tissue quality through light scattering materials, we investigated the feasibility of this technique to acquire Raman bands related to the fracture resistance of bone. Designed to maximize signals at different offsets, a SORS probe was used to acquire spectra from cadaveric bone with and without skin-like tissue phantoms attenuating the light. Autoclaving the lateral side of femur mid-shafts from 5 female and 5 male donors at 100 degrees C and again at 120 degrees C reduced the yield stress of cortical beams subjected to three-point bending. It did not affect the volumetric bone mineral density or porosity. Without tissue phantoms, autoclaving affected more Raman characteristics of the organic matrix when determined by peak intensity ratios, but fewer matrix properties depended on the three offsets (5 mm, 6 mm, and 7 mm) when determined by band area ratios. The cut-off in the thickness of the tissue phantom layers was-4 mm for most properties, irrespective of offset. Matching trends when spectra were acquired without phantom layers between bone and the probe, & nu;1PO43 ? /Amide III and & nu;1PO43%(proline + OHproline) were higher and lower in the non-treated bone than in the autoclaved bone, respectively, when the thickness of tissue phantom layers was 4 mm. The layers, however, caused a loss of sensitivity to autoclaving related changes in & nu;3CO3/& nu;1PO43 ? and crystallinity. Without advanced post-processing of Raman spectra, SORS acquisition through turbid layers can detect changes in Raman properties of bone that accompany a loss in bone strength.en_US
dc.identifier.doi10.1016/j.saa.2023.123240
dc.identifier.issn1386-1425
dc.identifier.issn1873-3557
dc.identifier.pmid37591015en_US
dc.identifier.pmid37591015
dc.identifier.scopus2-s2.0-85171807638
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.saa.2023.123240
dc.identifier.urihttps://hdl.handle.net/11492/7887
dc.identifier.volume303en_US
dc.identifier.wosWOS:001067593700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofSpectrochimica Acta Part A-Molecular and Biomolecular Spectroscopyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzkmusnmz
dc.subjectRaman spectroscopyen_US
dc.subjectBoneen_US
dc.subjectMineralen_US
dc.subjectCollagenen_US
dc.subjectStrengthen_US
dc.subjectAutoclavingen_US
dc.subjectFiber opticen_US
dc.titleAssessment of spatially offset Raman spectroscopy to detect differences in bone matrix qualityen_US
dc.typeArticle

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