Molecular characterization of Ditylenchus dipsaci on Onion in Turkey

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dc.authorid0000-0002-5836-7011en_US
dc.authorid0000-0002-3423-7095en_US
dc.authorid0000-0002-3264-2257en_US
dc.contributor.authorYavuzaslanoğlu, Elif
dc.contributor.authorSönmezoğlu, Özlem Ateş
dc.contributor.authorGenç, Nimet
dc.contributor.authorAkar, Zeki Mutlu
dc.contributor.authorTerzi, Begüm
dc.date.accessioned2019-12-06T21:15:33Z
dc.date.available2019-12-06T21:15:33Z
dc.date.issued2018
dc.departmentKMÜ, Teknik Bilimler Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.descriptionWOS:000428804400013en_US
dc.description.abstractDitylenchus dipsaci is a species complex including diploid and polyploid individuals. The onion race of D. dipsaci is a sensu stricto group and has a wide range of host spectrum. Identification of the D. dipsaci onion race is difficult using morphological and morphometrical methods. Species specific primers are mostly used in molecular approaches for identification of D. dipsaci populations. Fifty one morphologically selected Ditylenchus spp. populations from onion production areas in Turkey were subjected to molecular identification using four D. dipsaci species specific primer sets (PF1-PR1, PF2-PR2, DdpS1-rDNA2, DitNF1- rDNA2, H05-H06) targeting 5.8S and 18S rDNA, ITS1 and flanking ITS regions. Thirty nine percent of the nematode samples were positive with four primers tested, while four of the nematode samples gave specific bands with H05-H06 primers. Ditylenchus dipsaci sensu stricto was identified with specific primer sets in Adana, Hatay, Tekirdag, Bursa, Aksaray, Karaman, Eskisehir and Ankara provinces in Mediterranean, Trace, Aegean and Central Regions in Turkey.en_US
dc.description.sponsorshipTurkish Scientific and Technical Research Council (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215O468]en_US
dc.description.sponsorshipThis research has been financially supported by Turkish Scientific and Technical Research Council (TUBITAK) (Project No: 215O468).en_US
dc.identifier.citationYavuzaslanoğlu, E., Ateş, S. O., Genç, N., Akar, Z., Terzi, B. (2017). Molecular characterization of Ditylenchus dipsaci on Onion in Turkey. European Journal of Plant Pathology.
dc.identifier.doi10.1007/s10658-017-1366-7
dc.identifier.endpage200en_US
dc.identifier.issn0929-1873
dc.identifier.issn1573-8469
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85031929490
dc.identifier.scopusqualityQ1
dc.identifier.startpage195en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s10658-017-1366-7
dc.identifier.urihttps://hdl.handle.net/11492/2636
dc.identifier.volume151en_US
dc.identifier.wosWOS:000428804400013
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Sceince
dc.indekslendigikaynakScopus
dc.institutionauthorYavuzaslanoğlu, Elif
dc.institutionauthorSönmezoğlu, Özlem Ateş
dc.institutionauthorGenç, Nimet
dc.institutionauthorAkar, Zeki Mutlu
dc.institutionauthorTerzi, Begüm
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.journalEuropean Journal of Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOnionen_US
dc.subjectStemand Bulb Nematodeen_US
dc.subjectSpecies Specific Primeren_US
dc.subjectMolecular Characterizationen_US
dc.titleMolecular characterization of Ditylenchus dipsaci on Onion in Turkeyen_US
dc.typeArticle

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