Mühendislik Fakültesi, Biyomühendislik Bölümü, Makale Koleksiyonu

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https://hdl.handle.net/11492/864

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Quartz crystal microbalance modified with rhodamine-polyacrylonitrile nanofibers for acetone vapor sensing
(Elsevier Ltd, December 2025) Çapan, Rıfat; Çapan, İnci; Bayrakcı, Mevlüt
Rhodamine based polyacrylonitrile (PAN-RHE) electrospun nanofiber sensor was used to investigate for the vapor sensor application against acetone, ethanol, and benzene vapors at room temperature. Quartz crystal microbalance technique was employed to collect the time-dependent sensor response data which were analyzed for the determination of sensor parameters and for the investigation of the adsorption behavior between nanofiber and vapor molecules. The acetone vapor yielded the highest response with a best response with a sensitivity of 0.0243 Hz/ppm. The limit of detection and limit of quantification for acetone vapor were determined as 135.80 ppm and 411.52 ppm, respectively. Pseudo first-order and Elovich models were chosen to investigate adsorption dynamics. Pseudo first-order adsorption rate and Elovich desorption constant were calculated using time-dependent data. A possible hydrogen binding or dipole–dipole interaction between the vapor molecules and rhodamine and/or nitrile units of the PAN fiber chain was proposed as a sensor interaction mechanism.
Highly efficient photocatalyst based on Zn2-xBaxSnO4 alloying nanoparticles with enhanced photocatalytic activity
(Elsevier, 2025) Kamo, Alaa; Sönmezoğlu, Özlem Ateş; Sönmezoğlu, Savaş
In this study, an effective Zn2-xBaxSnO4 alloying nanoparticles were hydrothermally designed with a series of barium cation as alloying dopant and utilized as a photocatalyst to decompose the rhodamine B which causes harmful effects on humans such as allergic dermatitis, skin irritation, mutation, and cancer. It is noteworthy that the Zn1.988Ba0.012SnO4 alloying-based catalyst exhibited more than 99 % degradation in only 140 min in wastewater than barium-free ternary oxide. Moreover, the reaction rate of Zn1.988Ba0.012SnO4 alloying-based catalyst was enhanced to 0.032 min-1 compared with barium-free ternary oxide (0.0179 min-1). Based on scavenger trapping experiments, hydroxyl radicals are the main reactive oxygen species responsible for photocatalytic activity of Zn1.988Ba0.012SnO4 alloying-based catalyst. Besides, the photocatalytic rate was maintained 94.89 % after 5th cycle. This research not only provides a novel strategy for developing alloying-based catalysts but also unveils their potential in photoelectrochemistry and photocatalysis.
Functional properties of betalains: binding of hg2+ and cu2+ ions, sensor capabilities, and antimicrobial activity
(Wiley, 2025) Şen Arslan, Hülya; Yılmaz Altınok, Bahar
In this study, betalain extracts were obtained from prickly pear (PPE), dragon fruit (DFE), and red beet (RBE). The total betalain content of the extracts was determined to be 335.5 mg/L for PPE, 372.38 mg/L for DFE, and 466.21 mg/L for RBE, respectively. Fluorescence titrations with metal cations revealed PPE's highest intensity at 280 nm excitation, with DFE and RBE also responding, though less so. The fluorescence intensity for all extracts decreased significantly upon adding Hg2+ and Cu2+, indicating strong binding. PPE and DFE showed higher sensitivity and affinity for these ions than RBE. Absorption at 350 nm increased notably, along with visible color changes enabling potential 'naked-eye' detection. These extracts demonstrated high selectivity for Hg2+ and Cu2+, even with competing cations. Antibacterial tests showed significant inhibition against gram-positive and gram-negative bacteria. These findings, the extracts' strong spectral responses and anti-interference properties support their use as selective, sensitive sensors for environmental monitoring and analytical applications. Due to their inherent fluorescence properties, betalains have significant potential as biosensors.
High-throughput phenotyping of wheat root angle and coleoptile length at different temperatures using 3D-printed equipment
(2025) Aydın, Nevzat; Sönmez, Mesut Ersin; Güleç, Tuğba; Demir, Bedrettin; Alipour, Hadi; Türkoğlu, Aras
BackgroundInnovation in crop establishment is crucial for wheat productivity in drought-prone climates. Seedling establishment, the first stage of crop productivity, relies heavily on root and coleoptile system architecture for effective soil water and nutrient acquisition, particularly in regions practicing deep planting. Root phenotyping methods that quickly determine coleoptile lengths are vital for breeding studies. Traditionally, direct selection for root and coleoptile traits has been limited by the lack of suitable phenotyping methods, genetic and phenotypic complexity, and poor repeatability in sampling. In this study, we innovated a method utilizing 3D printing technology to measure root angle and coleoptile length in wheat seedlings. We evaluated seedlings from eight different wheat genotypes across varying temperatures and validated our findings through image processing techniques.ResultsThe analysis of variance in root architecture revealed significant differences among genotypes for root angle. Temperature treatments also significantly influenced shoot length, number of roots and total root length. The Tosunbey genotypes exhibited the highest root angle and the lowest root angle was observed in Altindane genotypes. Additionally, we observed that increasing the temperature led to an increase in seedling root length. Similarly, the coleoptile architecture analysis showed significant differences among genotypes in coleoptile length, leaf length, number of roots, and total root length. Temperature treatments and deep sowing applications significantly affected these traits as well. The Tosunbey and M & uuml;fitbey genotypes exhibited the longest coleoptile length, whereas the Nevzatbey genotype showed the shortest.ConclusionSelecting for a narrow root angle and a high number of seminal roots can result in deeper, more branched root systems. Furthermore, developing wheat genotypes with longer coleoptiles can enhance plant production and early vigor, particularly with deep sowing. Our method, using the eqiupments producing by 3D printing technology enables high-throughput phenotyping of wheat roots and coleoptiles, offering new insights into root and coleoptile system regulation at different temperature conditions. This method can be seamlessly integrated into breeding programs to enhance drought tolerance, rapidly phenotyping populations for root and coleoptile characteristics.
Green synthesis of iron oxide nanoparticles from extracts of Daucus carota subsp. sativus whole vegetable, peel, pomace, and juice and their application as antibacterial agents and Fenton-like catalysts
(Wiley, 2025) Kirtiş, Evrim Burcu Uncu; Koçak, Deniz Yiğit; Eldervis, Ukba; Tuna, Süheyla; Bayraç, Ceren
The green synthesis of iron oxide nanoparticles (Fe-NPs) with catalytic and antimicrobial activities from the extracts of different parts of Daucus carota subsp. sativus var. atrorubens Alef. was demonstrated in this study. The extracts of whole vegetable, peel, pomace, and juice acted as a reducing agent for the bio-reduction of Fe3+ into Fe-NPs. The synthesis of Fe-NPs was indicated by surface plasmon peaks within the range of 200-300 nm, as well as characteristic iron oxide bond formation in the region of 400-850 cm-1 using an ultraviolet-visible spectrophotometer and FT-IR spectrophotometer, respectively. The green synthesised Fe-NPs from the whole vegetable and its byproduct extracts had uniform quasi-spherical structures, with average particle sizes of 87.13, 80.95, 103.14, and 86.26 nm from whole vegetable extract, peel extract, pomace extract, and juice, respectively. All synthesised particles exhibited excellent Fenton-like catalytic activities on the discoloration of methylene blue best fitting the pseudo-first order kinetic model for Fe-NPs from whole vegetable extract, pomace extract, and juice and the pseudo-second order kinetic model for Fe-NPs from peel extract. In addition to catalytic activity, Fe-NPs showed antibacterial activity against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The antibacterial effects of Fe-NPs alone and their synergistic effects with antibiotics have been observed in both Gram-positive and Gram-negative bacteria.
Macroalgal (ulva compressa) silver nanoparticles: their characterization, cytotoxicity, and antibacterial applications
(Central Fisheries Research Inst, 2024) Armağan, Esra; Keskinateş, Mukaddes; Gümüş, Numan Emre; Aydın, Ziya; Yılmaz, Bahar; Bayrakcı, Mevlüt
In this study, an extract of Ulva compressa (UC), a green macroalgae, was used to synthesize biogenic silver nanoparticles (AgNPs). The formation of nanoparticles (NPs) was evaluated and confirmed by using ultraviolet-visible (UV-VIS) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and transmission electron microscopy (TEM) analyses. UV-VIS spectra showing absorption peaks at 450 nm confirmed the formation of AgNPs. The particles were crystalline in nature according to the XRD pattern. AgNPs formation was proved by the FT-IR spectrum of UC. TEM image showed that the average particle size was 4.55 nm. The antimicrobial and anticarcinogenic activity was evaluated for the synthesized AgNPs. AgNPs obtained from algae demonstrated antimicrobial activity against all bacteria including B. subtilis, E. faecalis, S. mutans, E. coli, S. aureus, and S. pyogenes. The NPs were shown to have anticarcinogenic activity on HEK 293, MCF-7, and HeLa (reducing viability by 25.42%, 46.42%, and 62.42%, respectively) cell cultures. These findings indicate that AgNPs can be useful medicinal compounds. Green synthesized NPs can be developed and utilized as anticancer agents in the treatment of various types of cancer, as well as their antimicrobial effects can be utilized to ensure the long-term sustainability of food in this study.
Assessing the impacts of titanium dioxide nanoparticles on seed germination and seedling growth in wheat
(Konya Teknik Univ, 2024) Ateş Sönmezoğlu, Özlem; Kamo, Alaa; Bozkaya, Büşra; Sönmezoğlu, Savaş
Wheat is the main food source for key nutrients in humans, hence any new research into boosting wheat quality and yield is vital. Recent advances in nanotechnology have made nanoparticles appropriate for use in agriculture. Titanium dioxide (TiO2) nanoparticles have a considerable impact on plants, but further research is required to make them commercially feasible. Herein, we evaluate the impact of TiO2 nanoparticles not only on the germination of seeds but also on the growth of wheat (Triticum aestivum L.) through soil application. The experimental findings reveal that TiO2 treatment at concentrations up to 2000 mg/L in seeds planted in Petri dishes has a favorable effect on wheat root and shoot length, whereas it has a suppressive effect at higher concentrations. As expected, a similar trend is observed for plant shoot length in the soil treatment with beneficial effects recorded up to a dosage of 60 mg/kg. In conclusion, the beneficial effects of TiO2 nanoparticles on seed germination and plant growth may have a big financial impact on horticulture, agriculture, and the energy industry-particularly on the manufacturing of biofuels.
Pre-harvest sprouting resistance in bread wheat: a speed breeding approach to assess dormancy qtl in backcross lines
(Mdpi, 2024) Ayık, Burak; Güleç, Tuğba; Aydın, Nevzat; Türkoğlu, Aras; Bocianowski, Jan
In this study, BC1F3:4 generation plants derived from the hybrid crosses of Rio Blanco x Nevzatbey, Rio Blanco x Adana99, and Rio Blanco x line 127 were used as experimental material. These hybrids incorporated QTLs associated with pre-harvest sprouting (PHS) resistance through molecular techniques. Key agronomic traits, including plant height, spike length, the number of grains per spike, grain weight, and physiological maturity, were evaluated in both greenhouse and growth room settings under accelerated growth (speed breeding) conditions. Results indicated that plants grown in the fully controlled greenhouse conditions exhibited superior agronomic performance compared with those cultivated in the growth room. Additionally, germination tests were conducted on each hybrid cross to identify genotypes exhibiting dormancy. The analysis revealed that 11 lines from the Rio Blanco x Nevzatbey combination, eight lines from Rio Blanco x Adana99, and six lines from Rio Blanco x Line 127 had notably low germination indices. Among the three hybrid families, the Rio Blanco x Nevzatbey BC1F3:4 hybrids demonstrated the lowest germination index (0.44). Furthermore, Rio Blanco itself recorded the lowest germination index under both greenhouse (0.02) and growth room (0.24) conditions. These findings suggest that environmental conditions exert a significant influence on the expression of dormancy in these genotypes, even when dormancy genes are present. The lines developed in this research have the potential to serve as elite material in breeding programs aimed at enhancing pre-harvest sprouting resistance.
Biosynthesis and characterization of silver nanoparticles mediated by cistus salviifolius L. and Ferula communis L. extracts and evaluation of their antioxidant, antibacterial, and cytotoxic potentials
(Pleiades Publishing Inc, 2024) Ulusu, Funda; Ulusu, Yakup
In this research, we employed a microwave-assisted green synthesis method utilizing extracts from Cistus salviifolius L. and Ferula communis L. to synthesize silver nanoparticles (AgNPs). Subsequently, we characterized the biosynthesized AgNPs, both Cs-AgNPs (derived from C. salviifolius) and Fc-AgNPs (derived from F. communis), through UV-Vis, FT-IR, XRD, SEM, and TEM analyses. The AgNPs exhibited a spherical shape with sizes averaging below 11 nm. To assess their antioxidant activity, we employed the DPPH method. Furthermore, we examined the antimicrobial potential of Cs-AgNPs and Fc-AgNPs against pathogenic bacterial strains, including Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli, using the disc diffusion assay and minimum inhibitory concentration. Additionally, we evaluated the cytotoxic effects of Cs-AgNPs and Fc-AgNPs on various cell lines, namely breast adenocarcinoma (MCF-7), colon carcinoma (HT-29), human keratinocytes (HaCaT), and embryonic kidney (HEK-293), employing the Alamar Blue assay. Our findings indicate that the biosynthesized Cs-AgNPs and Fc-AgNPs possess antioxidant, antibacterial, and anticancer activities. Therefore, the current study suggests that Cs-AgNPs and Fc-AgNPs exhibit potential for treating cancer and diseases caused by pathogenic microorganisms.
Long-duration type 1 diabetes is associated with deficient cortical bone mechanical behavior and altered matrix composition in human femoral bone
(Oxford Univ Press, 2024) Emerzian, Shannon R.; Unal, Mustafa; Brooks, Daniel J.; Wu, I-Hsien; Gauthier, John
Type 1 diabetes (T1D) is associated with an increased risk of hip fracture beyond what can be explained by reduced bone mineral density, possibly due to changes in bone material from accumulation of advanced glycation end-products (AGEs) and altered matrix composition, though data from human cortical bone in T1D are limited. The objective of this study was to evaluate cortical bone material behavior in T1D by examining specimens from cadaveric femora from older adults with long-duration T1D (>= 50 yr; n = 20) and age- and sex-matched nondiabetic controls (n = 14). Cortical bone was assessed by mechanical testing (4-point bending, cyclic reference point indentation, impact microindentation), AGE quantification [total fluorescent AGEs, pentosidine, carboxymethyl lysine (CML)], and matrix composition via Raman spectroscopy. Cortical bone from older adults with T1D had diminished postyield toughness to fracture (-30%, p = .036), elevated levels of AGEs (pentosidine, +17%, p = .039), lower mineral crystallinity (-1.4%, p = .010), greater proline hydroxylation (+1.9%, p = .009), and reduced glycosaminoglycan (GAG) content (-1.3%, p < .03) compared to nondiabetics. In multiple regression models to predict cortical bone toughness, cortical tissue mineral density, CML, and Raman spectroscopic measures of enzymatic collagen crosslinks and GAG content remained highly significant predictors of toughness, while diabetic status was no longer significant (adjusted R-2 > 0.60, p < .001). Thus, the impairment of cortical bone to absorb energy following long-duration T1D is well explained by AGE accumulation and modifications to the bone matrix. These results provide novel insight into the pathogenesis of skeletal fragility in individuals with T1D.
Ternary zinc-tin-oxide nanoparticles modified by magnesium ions as a visible-light-active photocatalyst with highly strong antibacterial activity
(Royal Soc Chemistry, 2024) Kamo, Alaa; Sönmezoğlu, Özlem Ateş; Sönmezoğlu, Savaş
Hospital-acquired infections (HAIs), especially nosocomial infections caused by antibiotic-resistant bacteria, are one of the most pressing health problems in all societies. Therefore, there is an urgent need to develop novel disinfection methods as alternatives to antibiotics to act against multidrug-resistant bacterial strains. Even though the photocatalytic disinfection phenomenon has been considered as a viable alternative compared to other proposed solutions, there is still a need to develop innovative functional materials for improving its efficacy under visible light to have a comparable impact to UV radiation. To boost the antibacterial efficacy under visible light, herein, we developed hydrothermally ternary zinc-tin-oxide (Zn2SnO4) nanoparticles modified with magnesium (Mg2+) ions at different doping ratios (0.5%, 1.0%, 1.5%, and 2.0%) as a photocatalytic disinfection agent and utilized it for the first time to kill Gram-negative (E. coli) and Gram-positive (S. aureus) pathogens that cause nosocomial infections. Moreover, we also explored how these materials interact with organic pollutants in the presence of visible light. Mg2+ cationic ions significantly enhanced the photocatalytic efficiency of ZTO nanoparticles under visible light to achieve 98% degradation of RhB dye in just 100 min, and rapidly produced numerous hydroxyl radicals as the main reactive oxygen species (ROS) responsible for the degradation, playing a key role in the nanoparticles impressive disinfection efficacy against these pathogenic bacteria. More importantly, Mg1.5@ZTO nanoparticles could effectively kill 99.76% of E. coli and 96.96% of S. aureus within only 1 h under visible light due to their smaller particle size, larger surface area, low recombination rate and greater ROS generation with oxygen vacancies. This research suggests that Mg-doped ZTO nanoparticles might be a viable and highly effective photocatalytic antibacterial agent candidate for future commercialization in healthcare and environmental applications.
Rhodamine-based electrospun polyacrylonitrile (pan) nanofiber sensor for the detection of chlorinated hydrocarbon vapors
(Amer Chemical Soc, 2024) Çapan, Rıfat; Çapan, İnci; Bayrakçı, Mevlüt
This study is the first report on the fabrication of polyacrylonitrile (PAN) nanofiber with rhodamine-based chemosensor (RHE) onto a mass-sensitive quartz crystal substrate using the electrospinning method and its sensing capability toward chlorinated hydrocarbons. Fabricated nanofiber webs via the electrospinning process are characterized by Fourier Transform Infrared (FTIR-ATR), Scanning Electron Microscopy, and Contact Angle measurement techniques, respectively. In order to investigate the vapor sensor properties, a Quartz Crystal Microbalance (QCM) system is employed to collect the real-time experimental data when the nanofiber sensor PAN-RHE is exposed to chlorinated hydrocarbons. Pseudo first-order and Elovich models are applied to elucidate the adsorption behavior. The morphological characterization proved smooth surface morphology without bead formation for all fibers with uniformity in the fiber skeleton. The average diameters of neat PAN and PAN nanofibers with RHE are found to be 449 and 790 nm, respectively. The nanofiber sensor PAN-RHE exhibits excellent sensing characteristics, including a high sensitivity of 0.0276 Hz/ppm, response and recovery times of 2-3 and 5-7 s, respectively, high selectivity for chloroform compared to other vapors tested, a limit of detection (LOD) of about 119.56 ppm, and a limit of quantification (LOQ) of about 362.31 ppm with a good reproducibility. The Pseudo-first-order adsorption rate and the Elovich desorption constants are determined as a function of different concentrations. The results obtained suggest that the QCM-based nanofiber sensor PAN-RHE shows great potential for the design of highly sensitive and selective chloroform sensors.
Unraveling the effects of strain-ınduced defect engineering on the visible-light-driven photodynamic performance of zn2sno4 nanoparticles modified by larger barium cations
(Amer Chemical Soc, 2024) Kamo, Alaa; Sönmezoğlu, Özlem Ateş; Sönmezoğlu, Savaş
Waterborne infections caused by pathogenic microorganisms represent serious health risks for humans. Ternary zinc-tin oxide nanoparticles have great potential as a cost-effective, environmentally friendly, and efficient candidate for waterborne infections; however, their photocatalytic and antibacterial effects are quite limited due to insufficient visible light absorption and rapid electron-hole recombination. Herein, barium-doped zinc stannate (Ba@ZTO) nanoparticles were synthesized by the hydrothermal method and used for the first time not only as antibacterial agents to prevent the spread of the harmful bacteria S. aureus and E. coli but also as photocatalysts to degrade the organic pollutant rhodamine B. Unexpectedly, Ba2+ ions exhibited compressive stress behavior instead of the predicted tensile stress when inserted into the ZTO crystal lattice, playing an active role in increasing oxygen vacancies within the crystal lattice and in the formation of hydroxyl radicals in the bulk solution and hydrogen peroxide (H2O2) radicals, significantly improving the photocatalytic and antibacterial properties. Strain-induced defects created by the insertion of larger barium ions into the ZTO lattice promote the increase of shallow traps for boosting photocatalytic/disinfection properties while suppressing deep-level traps that encourage nonradiative recombination. In essence, defect and strain engineering opens a promising route to achieve high disinfection efficiency by inducing larger cation ions under visible light in oxide-based materials.
Sensor Parameters and Adsorption Behaviour of Rhodamine-Based Polyacrylonitrile (PAN) Nanofiber Against Dichloromethane Vapour
(Elsevier, 2024) Çapan, Rıfat; Çapan, İnci; Bayrakçı, Mevlüt
This study is focused on the determination of sensor and adsorption parameters of rhodamine-based polyacrylonitrile (PAN) nanofiber during the dichloromethane vapour exposure due to a very limited investigation of the rhodamine-based PAN nanofiber sensor. Quartz crystal microbalance (QCM) measurement system is employed to monitor the sensor response to dichloromethane vapour. This nanofiber sensor demonstrates a highly sensitive, stable, reproducible response and concentration dependence behaviour. A good stability, with an excellent recovery occurred. Sensor parameters such as the limit of detection (153 ppm), the limit of quantification (465 ppm), the sensitivity (0.0215 Hz/ppm), response (2 s) and recovery (3 s) times are determined using QCM measurement results. Pseudo first-order and Elovich adsorption models have proved that dichloromethane vapours are adsorbed by this nanofiber sensor and the amount of vapour adsorption rate is increased when the concentration of dichloromethane vapour increases. Owing to its achieved sensor parameters, high adsorption feature and simple fabrication process with a low cost, this proposed sensing device is expected to be a promising alternative for monitoring dichloromethane vapour sensing application at room temperature.
Enhancing microalgae classification accuracy in marine ecosystems through convolutional neural networks and support vector machines
(Pergamon-Elsevier Science Ltd, 2024) Sönmez, Mesut Ersin; Gümüş, Numan Emre; Eczacıoğlu, Numan; Develi, Elif Eker; Yücel, Kamile; Yıldız Hüseyin Bekir
Accurately classifying microalgae species is vital for monitoring marine ecosystems and managing the emergence of marine mucilage, which is crucial for monitoring mucilage phenomena in marine environments. Traditional methods have been inadequate due to time-consuming processes and the need for expert knowledge. The purpose of this article is to employ convolutional neural networks (CNNs) and support vector machines (SVMs) to improve classification accuracy and efficiency. By employing advanced computational techniques, including MobileNet and GoogleNet models, alongside SVM classification, the study demonstrates significant advancements over conventional identification methods. In the classification of a dataset consisting of 7820 images using four different SVM kernel functions, the linear kernel achieved the highest success rate at 98.79 %. It is followed by the RBF kernel at 98.73 %, the polynomial kernel at 97.84 %, and the sigmoid kernel at 97.20 %. This research not only provides a methodological framework for future studies in marine biodiversity monitoring but also highlights the potential for real-time applications in ecological conservation and understanding mucilage dynamics amidst climate change and environmental pollution.
Wearable and implantable bioelectronic: Biosensing contact lens and applications
(Elsevier Science Sa, 2024) Saraç, Begüm; Yücer, Şeydanur; Sahin, Hilal; Ünal, Mustafa; Çiftçi, Fatih
The development of electronic contact lenses has been underway for over a decade by numerous research laboratories around the globe. The integration of electronics into contact lenses may facilitate medical capabilities, such as the measurement of intraocular pressure, the analysis of tears for glucose levels, and the assistance of individuals with impaired vision. While the science of creating smart contact lenses has advanced considerably, a significant challenge remains: the development of a power source capable of sustaining these devices for extended periods of time. The applications of wearable contact lenses offer a unified field of application in terms of both the electronics and biomaterials disciplines. This review article examines the diverse range of contact lens materials suitable for bioelectronics, encompassing PMMA, PET, PVA, hydrogel-based, silicon-based, and nanomaterial types, among others. It also explores studies investigating these materials in biosensing applications, including diabetic contact lenses, glucose sensing, drug delivery, and the detection of enzymatic biomarkers.
An Application of a Schiff-Base Type Reaction in the Synthesis of a New Rhodamine-Based Hg(II)-Sensing Agent (vol 29, pg 1349, 2019)
(Springer/Plenum Publishers, 2020) Çiçekbilek, Fulya; Altınok, Bahar Yılmaz; Bayrakcı, Mevlüt; Gezici, Orhan
[Abstract Not Available]
Accelerated breeding strategies for biochemical marker-assisted backcross breeding and mapping population development in bread wheat (Triticum aestivum L.)
(Springer, 2024) Aydın, Nevzat; Demir, Bedrettin; Akdağ, Halil; Gökmen, Sabri; Sayaslan, Abdulvahit; Bayraç, Ceren; Sönmez, Mesut Ersin
In order to rapidly adapt to the evolving climate and sustainably nourish the growing global population, plant breeders are actively investigating more efficient strategies to enhance crop yields. In this study, we present the development of a bread wheat mapping population and backcross breeding program, serving as a valuable genetic resource for mapping the effects of different alleles on trait performance. We employed innovative methodologies to rapidly introgress traits into the bread wheat cultivar. Specifically, we utilized two parents, including Tosunbey x Tahirova2000, in a recombinant inbred line population, in addition, a backcross strategy was applied with line 148 (obtained by crossing Tosunbey x Tahirova2000 with high gluten quality) as the recipient parent of the Nevzatbey cv., known for its awnless feature. The two most important applications of the rapid breeding method are extending the light period and breaking dormancy in early harvested seeds. Both applications were successfully implemented in our study. Our vegetation periods ranging from approximately 50-60 days. Additionally, an early genotype in our developed population was harvested in 40 days. Considering that the genotype underwent a 15-day vernalization period, the generation cycle, including vernalization, drying, and refrigeration, was completed in a total of 64 days. Notably, we employed not only biochemical markers for selection but also incorporated the rapid generation advance technology known as 'speed breeding', allowing us to develop BC5F1 within a span of two years. We posit that this approach proves instrumental in swiftly transferring genes for multiple target traits into adapted wheat cultivars or in pyramiding desirable traits within elite breeding material.
Evaluation of the effect of tartrazine on the offspring rats in an in vivo experimental model
(Wiley, 2024) Öztürk, Osman; Dikici, Yusuf; Gür, Öznur; Ocak, Mert; Doğanyiğit, Züleyha; Okan, Aslı; Söylemez, Evrim Suna Arıkan
Tartrazine, an azo dye prevalent in pharmaceuticals and food items, was investigated for its impact on fetal development, specifically examining visceral and skeletal abnormalities in rat offspring exposed to daily oral doses throughout pregnancy. Fourteen pregnant rats were randomly assigned to control and tartrazine groups (seven animals each), with tartrazine administered via oral gavage at 7.5 mg/kg throughout gestation. Offspring were categorized by gender for histopathological and genetic analysis of visceral structures. Bone quality and fracture resistance assessments involved micro-CT, Raman spectroscopy, and biomechanical testing. Results highlighted distinct internal organ tissue differences in the tartrazine group, notably increased hemorrhagic and inflammatory cell infiltration, degeneration, and vacuolization compared to controls. Gender-specific alterations in mRNA levels of IL-6, IL-1 beta, TNF-alpha, and TRPM2 genes (p < .001) were also noted. Moreover, tartrazine-exposed groups exhibited reduced trabecular thickness, bone volume, and significant alterations in bone matrix composition and quality alongside significant decreases in fracture resistance (p < 0.05). This study concludes that intrauterine exposure to tartrazine can result in adverse impacts on organ and bone development in rat offspring.
Yeni nesil DNA bazlı tek duvarlı karbon nanotüp biyosensörlerinin hazırlanması, karakterizasyonu ve NO molekülünün seçici olarak tespitinde kullanılması
(2023) Şen, Fatih; Bekmezci, Muhammed; Bayat, Ramazan; Tırı, Rıma Nour Elhouda
SWCNT yakın infrared bölgede (NIR) floresans emisyon verebilen bir karbon allotropudur. SWCNT?ler yüzeylerinin modifikasyonu, çeşitli analitler varlığında NIR floresansta değişikliklere yol açmaktadır. Yüzeyi modifiye edilmiş SWCNT?lerin bu özelliği tek molekül seviyesinde optik olarak tespitine olanak sağlamaktadır. Günümüzde tek molekül tespitinin yapılması, kanser gibi hastalıkların erken teşhisine olanak sağlamaktadır. Özellikle bir kanser belirteci olan nitrik oksit (NO) molekülünün düşük seviyelerde tespiti büyük bir önem taşımaktadır. Bu proje kapsamında, kiralliklerine göre ayrılan SWCNT?ların yüzeyleri, farklı uzunluktaki, Adenin-Timin, Guanin-Sitozin ve Guanin-Timin tek zincir DNA?lar ile modifiye edilmiştir. Hücre içinde en çok bulunan ve NO benzer yapıdaki moleküllerin kullanılması ile yapılan seçicilik haritaları sonucunda SWCNT (7,6)-AT30 biyosensör yapsının, NO molekülene karşı hassas olduğu görülmüştür. Oluşturulan sensör yapısının çeşitli karakterizasyon çalışmaları SWCNT (7.6)-AT30 yapısının oluştuğunu göstermektedir. Özelleştirilmiş tek molekül mikroskobunun kullanılması ile SWCNT (7.6)- AT15 kullanılarak yapılan çalışmalar soncunda, sensörün NO karşı Tayin Limiti (LOD) ve Tayin Sınırı (LOQ) değerleri sırasıyla, 1.24 µM ve 4.13 µM olarak bulunmuştur. A375 melanoma hücrelerinde yapılan sensör çalışması sonucunda, hücre içi NO tespiti gerçekleştirilmiş ve DAF-FM ile doğrulanmıştır.

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